Preparation of starch-based scaffolds for tissue engineering by supercritical immersion precipitation
Preparation of starch-based scaffolds for tissue engineering by supercritical immersion precipitation
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DOI：
10.1016/j.supflu.2008.12.008
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发表时间：
2009-06-01
影响因子：
3.9
通讯作者：
Reis, Rui L.
中科院分区：
文献类型：
--
作者：
Duarte, Ana Rita C.;Mano, Joao F.;Reis, Rui L.
本研究的目的是评估使用超临界流体技术制备淀粉基多孔基质的可能性。超临界浸没沉淀技术用于制备用于组织工程目的的淀粉和聚（L-乳酸）聚合物混合物的支架。在不同的操作条件下进行浸没沉淀实验，获得了高度多孔且互连的支架。对二氯甲烷和氯仿两种有机溶剂进行了测试，结果表明氯仿更适合该过程。评估了聚合物溶液浓度（5 至 20 wt%）、温度（35 至 55 摄氏度）和压力（100 至 200 bar）对 SPLA（50:50 wt%）膜形态、孔隙率和互连性的影响。所有测试条件均在有机溶剂和二氧化碳之间完全混溶的范围内。此外，还测试了具有不同淀粉-聚（L-乳酸）比例（30:70 wt%）的共混物。双连续结构的形成表明控制相转化的 L-L 分层过程是旋节线分解。 (C) 2008 Elsevier B.V. 保留所有权利。
The aim of this study was to evaluate the possibility of preparing starch-based porous matrixes using supercritical fluid technology. Supercritical immersion precipitation technique was used to prepare scaffolds of a polymeric blend of starch and poly(L-lactic acid) for tissue engineering purposes. Immersion precipitation experiments were carried out at different operational conditions and highly porous and interconnected scaffolds were obtained. Two organic solvents, dichloromethane and chloroform were tested, and from the results obtained chloroform was the more favourable for the process. The effect of polymer solution concentration (5 up to 20 wt%), temperature (35 up to 55 degrees C) and pressure (100 up to 200 bar) in the SPLA (50:50 wt%) membrane morphology, porosity and interconnectivity was evaluated. All the conditions tested were in the region of total miscibility between the organic solvent and carbon dioxide. Additionally, a blend with a different starch-poly(L-lactic acid) ratio (30:70 wt%) was tested. Bicontinuous structures were formed indicating that the L-L demixing process that governs the phase inversion is the spinodal decomposition. (C) 2008 Elsevier B.V. All rights reserved.