Oocyte metabolic function, lipid composition, and developmental potential are altered by diet in older mares.
Oocyte metabolic function, lipid composition, and developmental potential are altered by diet in older mares.
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DOI：
10.1530/rep-21-0351
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发表时间：
2022-04-01
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影响因子：
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通讯作者：
Carnevale EM
中科院分区：
文献类型：
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作者：
Catandi GD;LiPuma L;Obeidat YM;Maclellan LJ;Broeckling CD;Chen T;Chicco AJ;Carnevale EM
补充饲料是改善卵母细胞功能和体内发育潜力最可行的方法。在三个实验中，卵母细胞收集成熟，占主导地位的卵泡的老年人，以确定是否短期的膳食补充剂可以改变卵母细胞的代谢功能，脂质成分和发育潜力。在大约8周内，对照组饲喂干草（CON）或干草和谷物产品（COB）。接受治疗的马接受旨在促进马健康和胃肠道健康的补充剂、亚麻籽油以及脂肪酸和抗氧化剂支持的专有混合物（生殖支持补充剂（RSS）），旨在增加抗氧化活性和脂质氧化。针对使用额外抗氧化剂或改变n-3至n-6脂肪酸浓度的单独实验对RSS进行了修改。从补充RSS的卵母细胞与COB相比，有较高的基础耗氧量，表明较高的有氧代谢，和proportionalmore有氧厌氧代谢。在第二个实验中，在RSS补充之前（CON）和大约8周之后从相同的哺乳动物收集的卵母细胞具有显著降低的卵母细胞脂质丰度。在最后的实验中，将COB与RSS补充剂进行了比较，包括改良的RSS以比例减少n-3脂肪酸并增加n-6脂肪酸。无论脂肪酸含量如何，RSS的精子注射卵母细胞发育成囊胚的能力都高于COB。我们证明，短期的饮食补充可以直接影响卵母细胞的功能，在老年人，导致卵母细胞的代谢活性增加，脂质含量降低，并增加发育潜力。
Dietary supplementation is the most feasible method to improve oocyte function and developmental potential in vivo. During three experiments, oocytes were collected from maturing, dominant follicles of older mares to determine whether short-term dietary supplements can alter oocyte metabolic function, lipid composition, and developmental potential. Over approximately 8 weeks, control mares were fed hay (CON) or hay and grain products (COB). Treated mares received supplements designed for equine wellness and gastrointestinal health, flaxseed oil, and a proprietary blend of fatty acid and antioxidant support (reproductive support supplement (RSS)) intended to increase antioxidant activity and lipid oxidation. RSS was modified for individual experiments with additional antioxidants or altered concentrations of n-3 to n-6 fatty acids. Oocytes from mares supplemented with RSS when compared to COB had higher basal oxygen consumption, indicative of higher aerobic metabolism, and proportionately more aerobic to anaerobic metabolism. In the second experiment, oocytes collected from the same mares prior to (CON) and after approximately 8 weeks of RSS supplementation had significantly reduced oocyte lipid abundance. In the final experiment, COB was compared to RSS supplementation, including RSS modified to proportionately reduce n-3 fatty acids and increase n-6 fatty acids. The ability of sperm-injected oocytes to develop into blastocysts was higher for RSS, regardless of fatty acid content, than for COB. We demonstrated that short-term diet supplementation can directly affect oocyte function in older mares, resulting in oocytes with increased metabolic activity, reduced lipid content, and increased developmental potential.