Replicon typing of plasmids in Escherichia coli producing extended-spectrum β-lactamases
Replicon typing of plasmids in Escherichia coli producing extended-spectrum β-lactamases
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DOI：
10.1093/jac/dkn428
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发表时间：
2009-01-01
影响因子：
5.2
通讯作者：
Arlet, Guillaume
中科院分区：
文献类型：
--
作者：
Marcade, Geraldine;Deschamps, Catherine;Arlet, Guillaume
生产CTX-M-15和CTX-M-14广谱β -内酰胺酶（ESBLs）的大肠杆菌正在世界范围内传播。这项工作的目的是研究与ESBL类型相关的ESBL出现和传播的复制子。对125株产esbl的TEM、SHV和CTX-M大肠杆菌进行了分析。用PCR方法对携带ESBL的复制子和菌株的总质粒含量进行了与ESBL类型相关的复制子分型。然后将ESBL复制子与携带TEM-1或耐抑制剂TEM (IRT) β -内酰胺酶的大肠杆菌菌株的复制子含量进行比较。IncF质粒是我们收集的最常见的复制子，但没有携带TEM ESBL。TEM ESBLs中，除TEM-52基因优先携带inc1复制子外，67%的ESBLs携带在IncA/C复制子上。虽然CTX-M酶可以被各种复制子携带，但编码CTX-M-14和CTX-M-15 ESBLs的绝大多数基因都被IncF复制子携带，TEM-1和IRT β -内酰胺酶也是如此。由狭窄宿主范围内的IncF复制子携带的抗性基因很容易传播，因为这种复制子很好地适应了大肠杆菌。bla（TEM-1）和bla（CTX-M-15）也有这种情况，bla（CTX-M-14）也有这种情况。转位免疫似乎在扩散过程中起着重要作用。
Escherichia coli producing CTX-M-15 and CTX-M-14 extended-spectrum beta-lactamases (ESBLs) are spreading worldwide. The aim of this work was to investigate the replicons involved in the emergence and spread of ESBLs in relation to ESBL type.A collection of 125 TEM, SHV and CTX-M ESBL-producing E. coli strains was analysed. The replicons carrying the ESBLs and the total plasmid content of the strains have been characterized by PCR replicon typing in relation to the type of ESBL. The ESBL replicons were then compared with the replicon content of E. coli strains carrying TEM-1 or inhibitor-resistant TEM (IRT) beta-lactamases.IncF plasmids were the most frequently carried replicons in our collection, but none carried TEM ESBL. Of TEM ESBLs, 67% were carried on IncA/C replicons except for TEM-52 genes, which were carried preferentially on IncI1 replicons. Although CTX-M enzymes can be carried by various replicons, the great majority of genes encoding CTX-M-14 and CTX-M-15 ESBLs were carried by IncF replicons, as were TEM-1 and IRT beta-lactamases.Resistance genes borne by the narrow host-range IncF replicon spread readily as this replicon is well adapted to E. coli. This is observed for bla(TEM-1) and bla(CTX-M-15) and, to a lesser extent, for bla(CTX-M-14). Transposition immunity seems to play an important role in the diffusion process.