A histological evaluation on self-setting alpha-tricalcium phosphate applied in the rat bone cavity.
A histological evaluation on self-setting alpha-tricalcium phosphate applied in the rat bone cavity.
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发表时间：
2004
期刊：
影响因子：
14
通讯作者：
H. Hao;N. Amizuka;Kimimitsu Oda;Noritaka Fujii;H. Ohnishi;A. Okada;S. Nomura;T. Maeda
中科院分区：
文献类型：
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作者：
H. Hao;N. Amizuka;Kimimitsu Oda;Noritaka Fujii;H. Ohnishi;A. Okada;S. Nomura;T. Maeda
本研究旨在阐明自固化磷酸三钙骨替代物（BIOPEX）应用于大鼠股骨皮质骨腔的生物学效应。制备通过腔和骨髓的狭窄穿透以获得细胞来源。在实验组中，在第1天，薄的细胞层侵入到移植的BIOPEX和腔底部之间的狭窄空间中。从第5天到第10天，一系列抗酒石酸盐酸性磷酸酶（TRAP）反应性破骨细胞聚集在BIOPEX面向腔底的表面上，而许多碱性磷酸酶（ALP）阳性成骨细胞定位在BIOPEX对面的骨表面上。然而，在第20天，在BIOPEX上，成骨细胞位于破骨细胞附近，并将骨基质沉积在该材料上，这意味着破骨细胞和成骨细胞之间的偶联。术后第30天和第40天，新骨中存在少量BIOPEX颗粒残留物，具有密质骨轮廓。因此，BIOPEX被破骨细胞再吸收，并在后期通过破骨细胞和成骨细胞偶联的成骨细胞骨附着。总之，BIOPEX的使用提供了足够的骨再生和密质骨的轮廓。
This study aimed to elucidate the biological effects of a self-setting tricalcium phosphate bone substitute (BIOPEX) applied in rat femoral cortical bone cavities. Narrow penetrations through the cavity and bone marrow were prepared to obtain cellular sources. In the experimental group at day 1, a thin cell layer intruded into a narrow space between the grafted BIOPEX and the bottom of the cavity. From days 5 to 10, a range of tartrate-resistant acid phosphatase (TRAPase)-reactive osteoclasts accumulated on the surface of the BIOPEX facing the bottom of the cavity, whilst many alkaline phosphatase (ALPase)-positive osteoblasts were localized on the bone surface opposing the BIOPEX. However, at day 20, osteoblasts were localized neighboring the osteoclasts on the BIOPEX, and deposited bone matrices onto this material, implying a coupling between osteoclasts and osteoblasts. At days 30 and 40 post-operation, small remnants of BIOPEX particles were present in the new bone with a profile of compact bone. Thus, BIOPEX is resorbed by osteoclasts, and succeeded by osteoblastic bone apposition with a coupling of osteoclasts and osteoblasts at the later stage. In conclusion, the use of BIOPEX provides adequate bone regeneration with the profile of compact bone.