Secretion of N-terminal fragment of gamma-human atrial natriuretic polypeptide.
Secretion of N-terminal fragment of gamma-human atrial natriuretic polypeptide.
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γ-人心房钠尿多肽的N末端片段的分泌。
DOI：
10.1161/01.hyp.11.2_pt_2.i52
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发表时间：
1988
期刊：
影响因子：
8.3
通讯作者：
H. Imura
中科院分区：
文献类型：
--
作者：
H. Itoh;K. Nakao;M. Mukoyama;A. Sugawara;Y. Saito;N. Morii;T. Yamada;S. Shiono;H. Arai;H. Imura
为了阐明α-人心房利钠多肽(人心房利钠因子-[99-126])的前体激素--人心房利钠多肽(人心钠素-[1-126])的翻译后加工过程以及人心房利钠多肽在心脏中的分泌，我们建立了人心房利钠多肽N端序列的放射免疫分析方法和人心房利钠多肽的放射免疫分析方法。用放射免疫法测定正常人血浆中人心房利钠多肽-(1-25)和人心钠素α-(1-25)的水平为290+/-35.6pg/ml，同时测定的血浆α-人心钠素-(1-25)样免疫反应水平为20.9+/-2.8pg/ml，两者之间具有显著的相关性。高效凝胶渗透色谱分析表明，血浆γ-人心钠素-(1-25)样免疫反应是由一个相对分子质量为10000的组分组成，不存在α-人心钠素样免疫反应，而血浆α-人心钠素样免疫反应是由相对分子质量为3000的人心房利钠多肽组成。在心脏病患者中，血浆γ-人心钠素-(1-25)样免疫反应水平呈伴发性和渐进性升高，且与病情严重程度一致。两种免疫反应水平与右、左房压力呈显著正相关。
To elucidate the posttranslational processing of gamma-human atrial natriuretic polypeptide (human atrial natriuretic factor-[1-126]), which is a prohormone of alpha-human atrial natriuretic polypeptide (human atrial natriuretic factor-[99-126]), and the secretion of gamma-human atrial natriuretic polypeptide-derived peptides from the heart, we established a radioimmunoassay specific for the N-terminal sequence of gamma-human atrial natriuretic polypeptide, gamma-human atrial natriuretic polypeptide-(1-25), as well as a radioimmunoassay for alpha-human atrial natriuretic polypeptide. With the aid of the radioimmunoassays for gamma-human atrial natriuretic polypeptide-(1-25) and for alpha-human atrial natriuretic polypeptide, we detected 290 +/- 35.6 pg/ml of gamma-human atrial natriuretic polypeptide-(1-25)-like immunoreactivity in plasma from healthy humans, while the simultaneously determined plasma alpha-human atrial natriuretic polypeptide-like immunoreactivity level was 20.9 +/- 2.8 pg/ml. Correlation between the two values was significant. High performance gel permeation chromatographic analysis revealed that the plasma gamma-human atrial natriuretic polypeptide-(1-25)-like immunoreactivity was composed of a component (molecular weight, 10,000) without alpha-human atrial natriuretic polypeptide-like immunoreactivity, while the plasma alpha-human atrial natriuretic polypeptide-like immunoreactivity was composed of alpha-human atrial natriuretic polypeptide with a molecular weight of 3000. In patients with heart diseases, the plasma gamma-human atrial natriuretic polypeptide-(1-25)-like immunoreactivity level showed a concomitant and graded increase, with the plasma alpha-human atrial natriuretic polypeptide-like immunoreactivity level in agreement with the severity of the disease. There were significant positive correlations between the two immunoreactivity levels and right or left atrial pressure.(ABSTRACT TRUNCATED AT 250 WORDS)