The triplet energy of thymine in DNA
The triplet energy of thymine in DNA
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DOI：
10.1021/ja060651g
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发表时间：
2006-05-17
影响因子：
15
通讯作者：
Miranda, Miguel A.
中科院分区：
文献类型：
--
作者：
Bosca, Francisco;Lhiaubet-Vallet, Virginie;Miranda, Miguel A.
诺氟沙星（NFX）可光敏化DNA中胸腺嘧啶二聚体（T<>T）的形成，而其N（4 '）乙酰衍生物（ANFX）则无此作用。这从用T4内切核酸酶V酶处理后观察到的单链断裂和随后的凝胶电泳中是明显的。根据一组联苯和萘衍生物对三重态-三重态能量转移的猝灭，NFX和ANFX的三重态能量估计分别为273和268 kJ/mol。因此，DNA中胸腺嘧啶的三重态能量（即，光敏剂产生T（T）的值可以估计为270千焦/摩尔。
Norfloxacin (NFX) photosensitizes formation of thymine dimers (T<>T) in DNA, while its N(4‘) acetyl derivative (ANFX) does not. This is evident from the observation of single-strand breaks after enzymatic treatment with T4 endonuclease V and subsequent gel electrophoresis. The triplet energies of NFX and ANFX are estimated at 273 and 268 kJ/mol, respectively, on the basis of triplet−triplet energy transfer quenching by a set of biphenyl and naphthalene derivatives. Hence, the triplet energy of thymine in DNA (i.e., the value for a photosensitizer to produce T<>T) can be estimated at 270 kJ/mol.