Thrombin-stimulated uterine contractions in the pregnant and nonpregnant rat.
Thrombin-stimulated uterine contractions in the pregnant and nonpregnant rat.
复制标题
凝血酶刺激怀孕和非怀孕大鼠的子宫收缩。
DOI：
10.1016/s1071-5576(01)00121-6
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Saunders,T
中科院分区：
文献类型：
--
作者：
Phillippe,M;Elovitz,M;Saunders,T
目的：血液主动凝固过程中产生的凝血酶似乎是一种有效的子宫收缩激动剂;然而，这种对子宫平滑肌影响的机制尚不清楚。我们进行了研究，以确认凝血酶的子宫收缩作用，并确定是否前列腺素的生产过程中发挥作用的凝血酶或凝血blood.Methods的子宫收缩的影响：子宫收缩的研究进行了使用成年非妊娠和近足月妊娠大鼠。体外等长收缩研究使用吲哚美辛或溶剂（乙醇）预处理的子宫条，然后用凝血酶刺激。在体内收缩研究中，大鼠腹腔注射消炎痛或溶剂（乙醇），然后通过子宫腔内注射新鲜大鼠血液或凝血酶刺激。使用等长力传感器获得收缩数据，进行计算机数字化，针对自发活动进行标准化，并进行统计学分析。结果：体外子宫收缩实验表明，凝血酶和活性凝血均能显著增加子宫收缩的频率和强度。凝血酶刺激与体外PGF 2 α浓度增加54%相关;吲哚美辛（1 μM）预处理完全抑制PGF 2 α产生的增加。尽管PGF 2 α的产生受到抑制，但吲哚美辛预处理对凝血酶刺激的收缩活动没有抑制作用。在体内收缩研究进一步证实，吲哚美辛（2毫克/公斤）预处理对血液或凝血酶刺激的收缩activity.Conclusions没有影响：我们证实，凝血酶和凝血酶产生的积极凝血有强大的子宫收缩作用，在大鼠和前列腺素的生产没有发挥重要作用，凝血酶刺激的收缩。
Objective:Thrombin generated during the active clotting of blood appears to be a potent uterotonic agonist; however, the mechanism underlying this effect on uterine smooth muscle is not well understood. We performed studies to confirm the uterotonic effects of thrombin and to determine whether prostaglandin production plays a role during the uterotonic effects of thrombin or clotting blood.Methods:Uterine contraction studies were performed using adult nonpregnant and near-term pregnant rats. The in vitro isometric contraction studies used uterine strips pretreatd with indomethacia or vehicle (ethanol), which were then stimulated with thrombin. Forthe in vivo contraction studies, rats were pretreated with intraperitoneal injections of indomethacia or vehicle (ethanol) then stimulated by intraluminal injection of fresh rat blood or thrombin into the uterus. The contraction data were acquired using isometric force traducers, were computer digized, normalized for spontaneous activity, and statistically analyzed. Prostaglandin (PG) F2αwas measured using an enzyme-linked immunoassay.Results:The in vitro contraction studies demonstrated that both thrombin and actively clotting blood produce a significant increase in the frequency and intensity of uterine contractions. Thrombin stimulation was associated with a 54% increase in PGF2αconcentration in vitro; indomethacin (1 μM) pretreatment completely inhibited that increase in PGF2αproduction. Despite the suppression of PGF2αproduction, pretreatment with indomethacin had no inhibitory effect on thrombin-stimulated contractile activity. In vivo contraction studies further confirmed that indomethacin (2 mg/kg) pretreatment had no effect on blood- or thrombin-stimulated contractile activity.Conclusions:We confirmed that thrombin andthrombin produced by actively clotting blood had a robust uterotonic effect in the rat and that prostaglandin production did not play a significant role in thrombin-stimulated contractions.