Jejunal bypass stimulation of pancreatic growth and cholecystokinin secretion in rats: importance of luminal nutrients.
Jejunal bypass stimulation of pancreatic growth and cholecystokinin secretion in rats: importance of luminal nutrients.
复制标题
空肠旁路刺激大鼠胰腺生长和胆囊收缩素分泌：管腔营养的重要性。
作者：
Levan,VH;Liddle,RA;Green,GM
本文观察了空肠转流对大鼠胰腺生长和血浆胆囊收缩素（CCK）的影响。大鼠行空肠转流术或假手术。将空肠旁路大鼠进一步分为三组;一组接受部分水解的液体饮食（Vital）持续输注到旁路空肠中;第二组接受与胰蛋白酶混合的营养液并输注到旁路空肠中;第三旁路组不接受营养物或胰蛋白酶输注到空肠中。单纯空肠转流术在术后1周或2周时对胰腺生长或DNA含量无明显刺激作用。营养液注入旁路空肠刺激胰腺生长和DNA含量，胰腺重量和DNA含量的最大增幅分别为185%和181%，在两周。这与吸收后血浆CCK浓度的显著增加相吻合。将胰蛋白酶输注到旁路空肠中部分逆转了营养输注的作用。这些结果表明，从空肠排除胆胰液或胰腺蛋白酶不会导致CCK的最大释放，除非空肠接受管腔营养。有人建议，CCK释放从大鼠空肠自发发生在没有胰腺蛋白酶，并在旁路空肠腔营养增加血浆CCK和刺激胰腺生长，通过维持CCK的合成。
The effect of jejunal bypass on pancreatic growth and plasma cholecystokinin (CCK) was investigated in rats. Rats underwent bypass of jejunum or sham operation. Rats with jejunal bypass were further divided into three groups; one group received a continuous infusion of a partially hydrolysed liquid diet (Vital) into the bypassed jejunum; a second group received the nutrient solution mixed with trypsin and infused into the bypassed jejunum; the third bypass group did not receive infusion of nutrient or trypsin into the jejunum. Jejunal bypass alone did not significantly stimulate pancreatic growth or DNA content at one or two weeks postoperative. Infusion of nutrient solution into the bypassed jejunum stimulated pancreatic growth and DNA content, with maximal increases of 185% and 181% for pancreatic weight and DNA content, respectively, at two weeks. This coincided with significant increases in postabsorptive plasma CCK concentrations. Infusion of pancreatic proteases into the bypassed jejunum partially reversed the effects of nutrient infusion. These results suggest that exclusion of bile-pancreatic juice or pancreatic proteases from the jejunum does not lead to maximal release of CCK unless the jejunum receives luminal nutrients. It is proposed that CCK release from rat jejunum occurs spontaneously in the absence of pancreatic proteases, and that luminal nutrients in bypassed jejunum increase plasma CCK and stimulate pancreatic growth by maintaining synthesis of CCK.