Plastid position in Arabidopsis columella cells is similar in microgravity and on a random-positioning machine
Plastid position in Arabidopsis columella cells is similar in microgravity and on a random-positioning machine
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DOI：
10.1007/s004250000302
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发表时间：
2000-08-01
期刊：
影响因子：
4.3
通讯作者：
Kiss, JZ
中科院分区：
文献类型：
--
作者：
Kraft, TFB;van Loon, JJWA;Kiss, JZ
为了研究重力对植物结构和功能的影响，可能有必要去除 S 刺激。在地球上，生物学家使用了各种仪器，例如回转器，试图抵消重力的影响。在本研究中，测定了拟南芥 (L.) Heynh 根部小柱细胞中淀粉体的位置。幼苗在以下条件下生长：在地球上，在 1 rpm 的二维回转器上，在三维回转器（也称为随机定位机器或 RPM）上，以及在太空中（真正的微重力）。此外，还测量了这些重力处理对小柱细胞面积和质体面积的影响。就测量的参数而言，只有淀粉体位置受到重力处理的影响。质体位置在太空飞行和 RPM 条件之间没有显着差异，但在太空飞行和经典的二维回转器处理之间有显着差异。与中央小柱细胞相比，侧翼小柱细胞对重力变化更敏感。此外，在 RPM 上生长的幼苗的小柱细胞在其超微结构方面没有表现出有害影响，正如之前在二维回转器上生长的幼苗所报道的那样。这项研究支持这样的假设：RPM 提供了一种有用的失重模拟。
In order to study gravity effects on plant structure and function, it may become necessary to remove the S-stimulus. On Earth, various instruments such as clinostats have been used by biologists in an attempt to neutralize the effects of gravity. In this study, the position of amyloplasts was assayed in columella cells in the roots of Arabidopsis thaliana (L.) Heynh. seedlings grown in the following conditions: on Earth, on a two-dimensional clinostat at 1 rpm, on a three-dimensional clinostat (also called a random-positioning machiner or an RPM), and in space (true microgravity). In addition, the effects of these gravity treatments on columella cell area and plastid area also were measured. In terms of the parameters measured, only amyloplast position was affected by the gravity treatments. Plastid position was not significantly different between spaceflight and RPM conditions but was significantly different between spaceflight and the classical two-dimensional clinostat treatments. Flanking columella cells showed a greater susceptibility to changes in gravity compared to the central columella cells. In addition, columella cells of seedlings that were grown on the RPM did not exhibit deleterious effects in terms of their ultrastructure as has been reported previously for seedlings grown on a two-dimensional clinostat. This study supports the hypothesis that the RPM provides a useful simulation of weightlessness.