Gene quantification using real-time quantitative PCR: An emerging technology hits the mainstream
Gene quantification using real-time quantitative PCR: An emerging technology hits the mainstream
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DOI：
10.1016/s0301-472x(02)00806-8
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发表时间：
2002-06-01
影响因子：
2.6
通讯作者：
Ginzinger, DG
中科院分区：
文献类型：
--
作者：
Ginzinger, DG
最近大量使用实时Q-PCR的报告证明了这项技术从实验工具到科学主流的转变。实时Q-PCR的许多应用包括测量mRNA表达水平、DNA拷贝数、转基因拷贝数和表达分析、等位基因辨别和测量病毒滴度。实时Q-PCR的应用范围是巨大的，部分原因是低成本仪器和试剂的激增。实时Q-PCR的成功应用并非易事。然而，这篇评论将有助于引导读者了解可能限制这项技术有用性的变量。仔细考虑检测设计、模板制备和分析方法对于准确的基因定量是必不可少的。(C)2002年国际实验血液学学会。出版社：Elsevier Science Inc.
The recent flood of reports using real-time Q-PCR testifies to the transformation of this technology from an experimental tool into the scientific mainstream. Many of the applications of real-time Q-PCR include measuring mRNA expression levels, DNA copy number, transgene copy number and expression analysis, allelic discrimination, and measuring viral titers. The range of applications of real-time Q-PCR is immense and has been fueled in part by the proliferation of lower-cost instrumentation and reagents. Successful application of real-time Q-PCR is not trivial. However, this review will help guide the reader through the variables that can limit the usefulness of this technology. Careful consideration of the assay design, template preparation, and analytical methods are essential for accurate gene quantification. (C) 2002 International Society for Experimental Hematology. Published by Elsevier Science Inc.