Structure, chain conformation, and immunomodulatory activity of the polysaccharide purified from Bacillus Calmette Guerin formulation
Structure, chain conformation, and immunomodulatory activity of the polysaccharide purified from Bacillus Calmette Guerin formulation
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从卡介苗制剂中纯化的多糖的结构、链构象和免疫调节活性。
DOI：
10.1016/j.carbpol.2016.05.011
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发表时间：
2016-10-05
影响因子：
11.2
通讯作者：
Gao, Xiangdong
中科院分区：
文献类型：
--
作者：
Liu, Wei;Wang, Hong;Gao, Xiangdong
从卡介苗（BCG）多糖核酸注射液中分离纯化得到一种多糖，经FT-IR、HPAEC-PAD和NMR分析，其主要成分为α-D-（1 -> 4）-葡聚糖，并含有少量的岩藻糖和甘露糖。通过使用HPSEC-MALLS，测定的4、Mz和< s（2）> z（1/2）分别为1.320 × 10（5）g/mol、1.012 × 10（5）g/mol、2.139 × 10（5）g/mol和21.8 +/-3.2%nm。由（s（2））z（1/2）KMWv计算得到的v值为0.52 ± 0.01，首次阐明了BDP在0.9%NaCl水溶液中以无规卷曲形式存在。AFM和SEM结合刚果红试验也显示多糖为不规则的球状或卷曲状结构。此外，对RAW 264.7小鼠巨噬细胞的体外试验显示，BDP具有显著的免疫调节活性。(C)2016爱思唯尔有限公司版权所有。
A polysaccharide, coded as BDP, purified from the injection powder of Bacillus Calmette Guerin (BCG) polysaccharide and nucleic acid, was composed mainly of alpha-D-(1 -> 4)-linked glucan with (1 -> 6)-linked branches and trace amounts of fucose and mannose from the results of FT-IR, HPAEC-PAD and NMR spectrum. The 4,, Mz, and < s(2)> z(1/2) were determined to be 1.320 x 10(5) g/mol, 1.012 x 10(5) g/mol, 2.139 x 105 g/mol, and 21.8 +/- 3.2%nm by using HPSEC-MALLS, respectively. The v value from (s(2))z(1/2) KMWv was calculated to be 0.52 +/- 0.01, which firstly clarified that BDP existed as random coils in 0.9% NaCl aqueous solution. AFM and SEM combined with Congo -red test also revealed that the polysaccharide was irregular globular like or curly structure. Furthermore, in vitro tests on RAW264.7 murine macrophages cells revealed that BDP exhibited significant immunomodulatory activity. (C) 2016 Elsevier Ltd. All rights reserved.