COMPARISON OF EXTRACTION METHODS FOR THE RAPID-DETERMINATION OF ESSENTIAL OIL CONTENT AND COMPOSITION OF BASIL
COMPARISON OF EXTRACTION METHODS FOR THE RAPID-DETERMINATION OF ESSENTIAL OIL CONTENT AND COMPOSITION OF BASIL
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DOI：
10.21273/jashs.115.3.458
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发表时间：
1990-05-01
影响因子：
1.9
通讯作者：
SIMON, JE
中科院分区：
文献类型：
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作者：
CHARLES, DJ;SIMON, JE
从罗勒（Ocimumbasilicum）、罗勒（O. kilimandscharicum和O.采用溶剂萃取法、水蒸气蒸馏法和水蒸气蒸馏法测定挥发油的含量，并采用GC和GC/MS分析挥发油的组成。虽然从水蒸气蒸馏得到的精油产率始终高于水蒸气蒸馏，但从水蒸气蒸馏和水蒸气蒸馏回收的化合物数量相似。虽然两种方法的主要成分的相对浓度相似，但水蒸气蒸馏的绝对量较高。精油含量和组成因植物种类和植物部位而异。精油含量以O. basilicum和O.微甘菊使用新鲜或干燥样品以及使用75 g至10 g干燥植物组织的样品，在精油产率和主要成分的相对浓度方面没有观察到显著差异。虽然观察到水蒸气蒸馏和水蒸气蒸馏之间的微小差异，但这两种方法都能获得高产率和良好的精油成分回收率。水蒸馏是一种比蒸汽更快速、更简单的技术，可以在蒸汽无法到达的地方提取精油。
Essential oils were extracted from leaves, flowers, and stems of Ocimum basilicum, O. kilimandscharicum and O. micranthum by solvent extraction, hydrodistillation, and steam distillation for essential oil content and the oil analyzed by GC and GC/MS for composition. While the yield of essential oil was consistently higher from steam distillation than hydrodistillation, a similar number of compounds was recovered from both hydrodistillation and steam distillation . Though the relative concentration of the major constituents was similar by both methods, the absolute amounts were higher with steam distillation. Essential oil content and composition varied by plant species and plant part. Essential oil content was highest in flowers for O. basilicum and in leaves for O. micranthum. No significant differences were observed in essential oil yield and relative concentration of major constituents using fresh or dry samples and using samples from 75 g to 10 g of dry plant tissue. While minor differences between hydrodistillation and steam distillation were observed, both methods resulted in high yields and good recovery of essential oil constituents. Hydrodistillation is a more-rapid and simpler technique than steam and permits the extraction of essential oil where steam is not accessible.