Identification of additional rRNA fragments encoded by the Plasmodium falciparum 6 kb element
Identification of additional rRNA fragments encoded by the Plasmodium falciparum 6 kb element
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DOI：
10.1093/nar/25.2.438
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发表时间：
1997-01-15
影响因子：
14.9
通讯作者：
Morris, M
中科院分区：
文献类型：
--
作者：
Feagin, JE;Mericle, BL;Morris, M
在人疟原虫恶性疟原虫的6kb重复序列上，发现了类似于线粒体大小亚基rRNA的小片段。以前发现的rDNA序列包括rRNA的高度保守部分，这表明从它们衍生的片段rRNA能够结合成功能核糖体。然而，没有找到与其他预期rRNA区域相对应的序列。我们在此报告，在先前描述的13个rDNA区域中，有10个区域具有丰富的小转录本。另外还发现了10个转录本，这些转录本来自之前未知的含有基因的区域。其中5个已被鉴定为rRNA片段，包括与小亚基rRNA的5‘端和790环序列以及大亚基rRNA的sarcin/ricin环相对应的片段。证明了大多数先前描述的rDNA区域都有丰富的转录本，并且将新的转录本与传统rRNA的其他部分进行了鉴定，支持了这些小转录本由功能rRNA组成的假说。
Sequences similar to mitochondrial large and small subunit rRNAs are found as small scattered fragments on a tandemly reiterated 6 kb element in the human malaria parasite Plasmodium falciparum. The rDNA sequences previously identified include strongly conserved portions of rRNA, suggesting that fragmented rRNAs derived from them are able to associate into functional ribosomes. However, sequences corresponding to other expected rRNA regions were not found. We here report that 10 of the 13 previously described rDNA regions have abundant small transcripts. An additional 10 transcripts were found from regions not previously known to contain genes. Five of the latter have been identified as rRNA fragments, including those corresponding to the 5' end and 790 loop sequences of small subunit rRNA and the sarcin/ricin loop of large subunit rRNA. Demonstration that most of the previously described rDNA regions have abundant transcripts and the identification of new transcripts with other portions of conventional rRNAs provide support for the hypothesis that these small transcripts comprise functional rRNAs.