© 2012 Landes Bioscience. Do not distribute. Immunogenicity of a multi-epitope DNA vaccine against hantavirus
© 2012 Landes Bioscience. Do not distribute. Immunogenicity of a multi-epitope DNA vaccine against hantavirus
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发表时间：
2012
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通讯作者：
Chen Zhao;Ying Sun;Yujie Zhao;Si-liang Wang;Tongtong Yu;Feng Du;X. F. Yang;E. Luo
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作者：
Chen Zhao;Ying Sun;Yujie Zhao;Si-liang Wang;Tongtong Yu;Feng Du;X. F. Yang;E. Luo
肾综合征出血热（Hemorrhagic Fever with Renal Syndrome，HFRS）是由汉坦病毒（Hantaan virus，HTNV）、首尔病毒（Seoul virus，SEOV）、多布拉瓦病毒（Dobrava virus，DOBV）和普马拉病毒（Puumala virus）等汉坦病毒引起的一种严重流行性疾病。四种HFRS汉坦病毒中的三种，HTNV，SEOV和PUUV在中国发现。目前，没有有效的策略来降低感染风险。本研究构建了一种表达SEOV、HTNV和PUUV的25个糖蛋白表位的多表位嵌合DNA疫苗（简称SHP嵌合基因）。用SHP多表位嵌合DNA疫苗免疫BALB/c小鼠，导致体液和细胞应答显著增强。SHP疫苗DNA在许多器官中检测到，但不超过60 d。没有因整合而发生突变的风险。因此，SHP多表位嵌合DNA疫苗是一种潜在的有效和安全的抗SEOV，HTNV和PUUV感染的DNA疫苗。汉坦病毒是一种有包膜的病毒，具有三个反义单链RNA片段，分别命名为大（L）、中（M）和小（S）。基因组编码四种蛋白质：一种核衣壳蛋白（N），两种糖蛋白（Gc和Gn），和一种RNA。
Hemorrhagic fever with renal syndrome (HFRS) is a severe epidemic disease caused by hantaviruses including Hantaan virus (HTNV), Seoul virus (SEOV), Dobrava virus (DOBV) and Puumala virus. Three of the four HFRS hantaviruses, HTNV, SEOV, and PUUV are found in China. Currently, there is no effective strategy available to reduce infection risk. In this study, we constructed a multi-epitope chimeric DNA vaccine that encodes expressing 25 glycoprotein epitopes from SEOV, HTNV and PUUV (designated as SHP chimeric gene). Vaccination of BALb/c mice with SHP multi-epitope chimeric DNA vaccine led to a dramatic augmentation of humoral and cellular responses. The SHP vaccine DNA was detected in many organs but not for more than 60 d. There was no risk of mutation due to integration. Thus, the SHP multi-epitope chimeric DNA vaccine is a potential effective and safe DNA vaccine against infection by SEOV, HTNV, and PUUV. Hantaviruses are enveloped viruses with three segments of antisense single-stranded RNA designated as large (L), medium (M), and small (S). The genome encodes four proteins: a nucleocapsid protein (N), two glycoproteins (Gc and Gn), and an RNA