On the Mechanism of Membrane Protein Crystallization in Lipidic Mesophases
On the Mechanism of Membrane Protein Crystallization in Lipidic Mesophases
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DOI：
10.1021/cg800693r
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发表时间：
2008-12-01
影响因子：
3.8
通讯作者：
Caffrey, Martin
中科院分区：
文献类型：
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作者：
Caffrey, Martin
用于膜蛋白结晶的介观方法已被证明适用于一系列不同类型的蛋白质。该方法包括将目标蛋白重组为双连续脂类中间相的双层，然后通过添加沉淀剂进行诱导相分离。对于介观结晶如何在分子水平上发生，已经提出了一个机制。然而，这一假说的各个方面还没有完全发展起来。在这里，通过考虑相关但更简单的系统和过程的行为来检查和扩展这些行为，在这些系统和过程中，行为模式是在实验中被理解和建立的。通过建立这一系列的模型，行为良好的系统获得了在膜蛋白的介观结晶发生过程中发生的更完整的观点。除了基本的理论之外，还探讨了该方法的一些更实际的方面，并建立了它们在假设中的基础。这种治疗方法建议进行大量实验，以进一步检验和发展这一假说。通过破译潜在的分子机制，更清晰的洞察力出现在实践膜蛋白结晶的合理方法上。
The in meso method for crystallizing membrane proteins has been shown to work with a range of different protein types. The method involves reconstituting the target protein into the bilayer of a bicontinuous lipid mesophase followed by an induced phase separation brought on by the addition of a precipitant. A mechanism has been proposed for how in meso crystallogenesis comes about at a molecular level. However, aspects of the hypothesis are not fully developed. These are examined and expanded on here by considering the behavior of related but simpler systems and processes where the mode of action is understood and founded in experiment. By building up from this series of model, well behaved systems a more complete view of what takes place during the in meso crystallogenesis of membrane proteins is obtained. In addition to the underlying theory, some of the more practical aspects of the method are explored and their basis in the hypothesis is established. This treatment suggests a host of experiments to further test and develop the hypothesis. By deciphering the underlying molecular mechanism, clearer insights emerge for practicing a rational approach to membrane protein crystallogenesis.