The macrophage heterogeneity:: Difference between mouse peritoneal exudate and splenic F4/80+ macrophages
The macrophage heterogeneity:: Difference between mouse peritoneal exudate and splenic F4/80+ macrophages
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DOI：
10.1002/jcp.20732
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发表时间：
2006-11-01
影响因子：
5.6
通讯作者：
Zhao, Yong
中科院分区：
文献类型：
--
作者：
Liu, Guangwei;Xia, Xue-Pei;Zhao, Yong
从各种组织中分离的巨噬细胞表现出细胞形状、表面标志物表达以及代谢和功能活性的差异。然而，在不同组织中表达相同标志物的巨噬细胞的异质性尚未完全解决。在本研究中，小鼠F4/80（+）腹腔渗出液巨噬细胞（PEM）和脾巨噬细胞（SPM）在大多数方面相似。但F4/80（+）SPM中表达CD 80、CD 40、MHC-II、TILR 2或TILR 4的细胞百分比显著高于PEM，而表达CD 86（+）的细胞百分比显著低于PEM。在脂多糖（LPS）刺激后，F4/80（+）SPM表达的CD 86、CD 40或MHC-II水平显著高于F4/80（+）PEM，但不表达CD 11 c、CD 80、CD 54或CD 23。双光子显微镜和流式细胞仪检测表明，F4/80（+）SPM对鸡红细胞和异基因T细胞的非调理吞噬能力明显低于PEM。当与LPS或allo-T细胞一起培养时，SPM产生的NO显著高于PEM。此外，SPM表现出比PEM更强的免疫原性，如通过刺激T细胞增殖、迟发型超敏反应和IFN-γ产生的能力所确定的。这些数据显示了SPM和PEM在表型、吞噬功能和免疫原性方面的差异，这可能为我们更好地了解脾和腹腔巨噬细胞的免疫反应提供重要信息。
Macrophages isolated from various tissues manifest differences in cell shape, the expression of surface markers, as well as metabolic and functional activities. However, the heterogeneity of macrophages expressing the same marker in different tissues has not been fully addressed. In the present study, mouse F4/80(+) peritoneal exudate macrophages (PEMs) and splenic macrophages (SPMs) appeared similar in most respects. But the percentages of cells expressing CD80, CD40, MHC-II, TILR2, or TILR4, but not CD11c, CD54, or CD23, in freshly isolated F4/80(+) SPMs were significantly higher than those in PEMs, whereas the levels of CD86(+) cells in F4/80(+) SPMs were markedly lower than those in PEMs. After lipopolysaccharide (LPS) stimulation, F4/80(+) SPMs expressed significantly higher levels of CD86, CD40, or MHC-II than F4/80(+) PEMs, but not CD11c, CD80, CD54, or CD23. F4/80(+) SPMs had remarkably lower non-opsonic phagocytotic capacity against chicken RBCs or allo-T cells than PEMs as determined by two-photon microscopes and flow cytometry. SPMs produced markedly more NO than PEMs when cultured with LPS or allo-T cells. Furthermore, SPMs exhibited stronger immunogenicity than PEMs, as determined by the ability to stimulate T cell proliferation, delayed type hypersensitivity, and IFN-gamma production. The data showed the differences between SPMs and PEMs with regard to the phenotypes, phagocytosis, and immunogenicity, which may offer important information for us to better understand the distinguished immune responses of macrophages in spleens and the peritoneal cavity.