Effects of chlorantraniliprole exposure on detoxification enzyme activities and detoxification-related gene expression in the fat body of the silkworm, Bombyx mori
Effects of chlorantraniliprole exposure on detoxification enzyme activities and detoxification-related gene expression in the fat body of the silkworm, Bombyx mori
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DOI：
10.1016/j.ecoenv.2019.03.074
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发表时间：
2019-07-30
影响因子：
6.8
通讯作者：
Li, Bing
中科院分区：
文献类型：
--
作者：
Mao, Tingting;Li, Fanchi;Li, Bing
氯虫苯甲酰胺（CAP）可诱导昆虫肌肉释放过量钙，导致肌肉麻痹直至死亡，其在农田中的残留可引起家蚕（B）中毒。mori），导致结茧失败。目前还没有研究CAP暴露对B中解毒酶活性和解毒相关基因表达的影响的报道。森。本研究采用CAP浸叶法处理桑葚，然后用B.用污染的桑葚连续饲喂桑幼虫。此外，还对B脂肪体中解毒酶活性及解毒相关基因的表达进行了研究。森被检查。结果表明，CAP暴露后24 h，P450和GST酶活性均显著升高，其中P450酶反应最快。与对照组相比，CarE酶活性在24 h内升高，随后下降。PI 3 K/Akt/CncC信号通路中的关键基因（PI 3 K、PDK、Akt、CncC和Keap 1）在mRNA水平的表达显著增加。Western blotting分析显示Akt在蛋白水平受到抑制，导致Keap 1表达减少，CncC表达增加。这些结果表明，B脂肪体中存在PI 3 K/Akt/CncC信号通路。家蚕对CAP的暴露产生反应，调节下游解毒酶的表达，从而增强了B的解毒能力。玛丽。
Chlorantraniliprole (CAP) can induce excessive calcium release from muscle of insects, causing muscle paralysis until death, and its residues in farmland can cause poisoning in Bombyx mori (B. mori), resulting in the failure of cocooning. No reports have investigated the effects of CAP exposure on detoxification enzyme activities and detoxification-related gene expression in B. mori. In the present study, we treated mulberry leaves with CAP by the leaf-dipping method, and then B. mori larvae were continuously fed with the polluted mulberry leaves. Moreover, the detoxification enzyme activities and the expressions of detoxification-related genes in the fat body of B. mori were examined. The results showed that at 24 h after CAP exposure, the activities of P450 and GST enzymes were all significantly increased, with P450 enzymes responding fastest. CarE enzyme activity was up regulated in 24 h, and then it was decreased compared with the control group. Furthermore, the expressions of the key genes in the PI3K/Akt/CncC signaling pathway (PI3K, PDK, Akt, CncC and Keap1) at the mRNA were significantly increased. Western blotting analysis revealed that Akt was inhibited at the protein level, resulting in decreased expression of Keap1 and increased expression of CncC. These results indicated that the PI3K/Akt/CncC signaling pathway in the fat body of B. mori responded to CAP exposure and regulated the expressions of downstream detoxification enzymes, thus enhancing the detoxifying capability of B. mari.