CLONING AND CHARACTERIZATION OF A CDNA-ENCODING THE RAT-BRAIN GLUCOSE-TRANSPORTER PROTEIN
CLONING AND CHARACTERIZATION OF A CDNA-ENCODING THE RAT-BRAIN GLUCOSE-TRANSPORTER PROTEIN
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DOI：
10.1073/pnas.83.16.5784
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发表时间：
1986-08-01
影响因子：
11.1
通讯作者：
ROSEN, OM
中科院分区：
文献类型：
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作者：
BIRNBAUM, MJ;HASPEL, HC;ROSEN, OM
针对人红细胞葡萄糖转运蛋白的抗体鉴定出重组的。lambda。用免疫选择的成年大鼠脑多体RNA制备cDNA文库中的gt11噬菌体。该cDNA预测了一个492个氨基酸的蛋白，与人类肝癌己糖载体具有97.6%的同源性。转运蛋白mRNA的组织分布与免疫可识别蛋白的组织分布和转运活性相同，但在肝脏中，高水平的转运与很少或没有转运蛋白mRNA或蛋白质相关。通过斑点杂交分析，来自胰岛素反应组织和非反应组织的mRNA无法区分。这些数据表明，一种与基因无关的蛋白质在正常肝脏中负责己糖的运输。
Antibody raised against the human erythrocyte glucose transporter identified a recombinant .lambda.gt11 bacteriophage in a cDNA library prepared from immunoselected polysomal RNA from adult rat brain. The cDNA predicts a 492-amino acid protein that demonstrates 97.6% identity to the human hepatoma hexose carrier. The tissue distribution of the transporter mRNA is identical to that of immunologically identifiable protein and transport activity, except in liver in which high levels of transport are associated with little or no transporter mRNA or protein. As assayed by blot-hybridization analysis, mRNA from insulin-responsive and nonresponsive tissues are indistinguishable. These data suggest that a genetically unrelated protein is responsible for hexose transport in normal liver.