In vitro hemocompatibility testing of UV-modified hyaluronan hydrogels
In vitro hemocompatibility testing of UV-modified hyaluronan hydrogels
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DOI：
10.1016/j.biomaterials.2005.08.008
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发表时间：
2006-03-01
期刊：
影响因子：
14
通讯作者：
Ramamurthi, A
中科院分区：
文献类型：
--
作者：
Amarnath, LP;Srinivas, A;Ramamurthi, A
基于交联透明质酸（HA）的水凝胶（hylans）是用于血管组织工程应用的潜在良好生物材料，因为它们具有高度的非抗原性和免疫原性。为了促进表面内皮化，这对血管展开至关重要，我们用低波长UV光照射凝胶表面。该过程使光滑的凝胶表面微观纹理化以提供用于细胞锚定的位点，并引起天然长链HA的有限断裂，产生引起增强的细胞应答的较小片段。在目前的体外研究中，我们评估了紫外线照射对短期的影响。（< 45 min）hylan凝胶与人血细胞之间的相互作用尽管相对于未修饰的（U）hylans，经辐照的（UV）hylans的降低的亲水性引起更大的血管细胞反应，血小板沉积不受影响，并且与胶原涂覆的玻璃对照相比低得多。粘附的血小板呈圆形或轻度伪足状，不表达活化标志物P-选择素。两种类型的凝胶诱导相同的，最小的血小板释放使用血小板因子4 ELISA测定，并同样推迟内源性和外源性凝血途径。这两种类型的凝胶诱导接触激活的结合，但不是血浆相因子XII相对于对照水平升高。溶血率也相同，且在可接受的标准范围内。我们的结论是，紫外线处理的hylans，有益于改善表面内皮化，不损害其短期的血液相容性，至关重要的，他们作为血管植入材料的使用。(c)2005爱思唯尔有限公司保留所有权利。
Hydrogels (hylans) based on cross-linked hyaluronan (HA) are potentially good biomaterials for vascular tissue engineering applications because they are highly non-antigenic and -immunogenic. To facilitate surface endothelialization, vital to vascular deployment, we irradiated the gel surface with low wavelength UV light. This process micro-textures the smooth gel surface to provide sites for cell anchorage and causes limited scission of native long-chain HA yielding smaller fragments that elicit an enhanced cell response. In the current in vitro study, we assessed the effects of UV irradiation on the short-term (< 45 min) interaction between hylan gels and human blood cells (RBCs, platelets) and coagulation proteins at physiologic temperature.Although the lowered hydrophilicity of irradiated (UV) hylans elicited greater vascular cell response relative to unmodified (U) hylans, platelet deposition was unaffected and much lower compared to collagen-coated glass controls. The adhered platelets were rounded or mildly pseudopodic and did not express p-selectin, an activation marker. Both gel types induced identical, and minimal platelet release as measured using an platelet factor 4 ELISA, and identically deferred the intrinsic and extrinsic coagulation pathways. Both gel types induced elevated levels of contact activation of bound, but not plasma-phase factor XII relative to controls. Hemolysis rates were also identical and within accepted standards. We conclude that UV-treatment of hylans, useful to improve surface endothelialization, does not compromise their short-term hemocompatibility, vital to their use as vascular implant materials. (c) 2005 Elsevier Ltd. All rights reserved.