Effects of Osiris9a on Silk Properties in Bombyx mori Determined by Transgenic Overexpression
Effects of Osiris9a on Silk Properties in Bombyx mori Determined by Transgenic Overexpression
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转基因过表达测定 Osiris9a 对家蚕丝特性的影响
DOI：
10.3390/ijms21051888
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发表时间：
2020-03-01
影响因子：
5.6
通讯作者：
Liu, Chun
中科院分区：
文献类型：
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作者：
Cheng, Tingcai;Zhang, Xia;Liu, Chun
Osiris是一个昆虫特异性基因家族，在发育、表型多态性和保护等方面具有多种生物学功能。在家蚕中，我们以前已经确定了25个Osiris基因，这些基因在几种昆虫中具有高度的进化保守性和显着的同线性。家蚕Osiris9a（BmOsi9a）仅在丝腺中表达，特别是在中间丝腺（MSG）中。然而，BmOsi9a的生物学功能仍然未知。在这项研究中，我们过表达BmOsi9a在丝腺的生殖系转基因表达。BmOsi9a不仅在MSG中过表达，而且在后丝腺（PSG）中也过表达。有趣的是，BmOsi9a可以在MSG中分泌到管腔中，但在PSG中不能。在丝纤维中，过表达的BmOsi9a与MSG中的Sericin1相互作用，如通过免疫共沉淀测定所证实的。BmOsi9a的过表达改变了丝纤维的二级结构和结晶度，从而改变了机械性能。这些结果提供了深入了解丝蛋白分泌和丝纤维形成的机制。
Osiris is an insect-specific gene family with multiple biological roles in development, phenotypic polymorphism, and protection. In the silkworm, we have previously identified twenty-five Osiris genes with high evolutionary conservation and remarkable synteny among several insects. Bombxy mori Osiris9a (BmOsi9a) is expressed only in the silk gland, particularly in the middle silk gland (MSG). However, the biological function of BmOsi9a is still unknown. In this study, we overexpressed BmOsi9a in the silk gland by germline transgene expression. BmOsi9a was overexpressed not only in the MSG but also in the posterior silk gland (PSG). Interestingly, BmOsi9a could be secreted into the lumen in the MSG but not in the PSG. In the silk fiber, overexpressed BmOsi9a interacted with Sericin1 in the MSG, as confirmed by a co-immunoprecipitation assay. The overexpression of BmOsi9a altered the secondary structure and crystallinity of the silk fiber, thereby changing the mechanical properties. These results provide insight into the mechanisms underlying silk proteins secretion and silk fiber formation.