A novel simplistic fabrication technique for cranial epidural electrodes for chronic recording and stimulation in rats.
A novel simplistic fabrication technique for cranial epidural electrodes for chronic recording and stimulation in rats.
复制标题
一种用于大鼠慢性记录和刺激的颅硬膜外电极的新颖简单制造技术。
DOI：
10.1016/j.jneumeth.2018.10.036
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发表时间：
2019
影响因子：
3
通讯作者：
Russell C
中科院分区：
文献类型：
--
作者：
Russell C
背景：对神经调节和记录工具的需求导致了描述适合于神经记录的内部设备和附件制造技术的出版物的激增。然而，这些制作方案中的许多都使用了生物实验室不常见的设备，从而限制了研究人员使用商业替代。新方法：我们开发了一种简单但坚固的植入式刺激表面电极，可以在所有湿台实验室中制造。结果：雌性SD大鼠在其运动皮质的前后肢区域接受了硬膜外植入电极。刺激运动皮质成功地诱发了前肢和后肢的运动输出。该装置还能够记录硬膜外刺激脊髓后运动皮质的表面电位。与现有方法相比：刺激运动皮质时，往往将僵硬的不锈钢或铜线粗略地塞在颅底，定位精度很低。然而，商业上可用的设备使用毛刺孔和螺丝电极。我们的新电极设计为我们提供了以前所没有的立体定位精度。结论：我们开发了一种慢性植入电极，能够在所有湿实验室中制造，具有坚固、多功能和足够的电敏性，可以长期长期使用。简单而多功能的电极设计提供了科学、经济和伦理上的好处。
Background:The demand for neuromodulatory and recording tools has resulted in a surge of publications describing techniques for fabricating devices and accessories in-house suitable for neurological recordings. However, many of these fabrication protocols use equipment which are not common to biological laboratories, thus limiting researchers to the use of commercial alternatives.New method:We have developed a simple yet robust implantable stimulating surface electrode which can be fabricated in all wet-bench laboratories.Results:Female Sprague-Dawley rats received epidural implantation of the electrodes over the fore and hind limb areas of their motor cortex. Stimulation of the motor cortex successfully evoked fore- and hind limb motor outputs. The device was also able to record surface potentials of the motor cortex following epidural stimulation of the spinal cord.Comparisons with existing methods:For stimulation of the motor cortex, often stiff stainless or copper wires are roughly tucked underneath the skull, with little accuracy of localization. While, commercially available devices utilize burr holes and screw electrodes. Our new electrode design provides us stereotaxic accuracy that was not previously available.Conclusion:We developed a chronic implantable electrode capable of being fabricated in all wet-labs, are robust, versatile and electrically sensitive enough for long-term chronic use. The simple and versatile electrode design provides scientific, economical and ethical benefits.