Spore coat proteins of Dictyostelium discoideum are packaged in prespore vesicles.
Spore coat proteins of Dictyostelium discoideum are packaged in prespore vesicles.
复制标题
盘基网柄菌的孢子外壳蛋白包装在前孢子囊泡中。
DOI：
10.1016/0012-1606(83)90293-2
复制
发表时间：
1983
影响因子：
2.7
通讯作者：
Loomis,WF
中科院分区：
文献类型：
--
作者：
Devine,KM;Bergmann,JE;Loomis,WF
先前的研究表明盘状网柄藻孢子外壳蛋白存在于游动蛞蝓后部的前孢子细胞和成熟孢子的外壳中。前孢子囊泡，通过形态学和抗D染色鉴定。mucoroidespore血清，也定位于迁移蛞蝓的后部区域。使用特异性的孢子外壳蛋白的抗血清，我们表明，孢子外壳蛋白被包装在prepore囊泡。它们作为可通过变性解离的复合物存在于囊泡中。抗D抗体毛霉孢子血清与全孢子提取物中的至少五种蛋白质反应，包括孢子外壳蛋白SP96和SP70。
Previous studies have shown thatDictyostelium discoideumspore coat proteins are found in prespore cells, which are localized to the posterior region of migrating slugs, and in the coats of mature spores. Prespore vesicles, identified by morphology and by staining with anti-D. mucoroidesspore serum, are also localized in the posterior region of migrating slugs. Using antisera specific to the spore coat proteins, we show that the spore coat proteins are packaged in prespore vesicles. They are present in the vesicles as a complex which can be dissociated by denaturation. The anti-D. mucoroidesspore serum reacts with at least five proteins in whole spore extracts including the spore coat proteins SP96 and SP70.