Cydia pomonella granulovirus Genotypes Overcome Virus Resistance in the Codling Moth and Improve Virus Efficiency by Selection against Resistant Hosts
Cydia pomonella granulovirus Genotypes Overcome Virus Resistance in the Codling Moth and Improve Virus Efficiency by Selection against Resistant Hosts
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DOI：
10.1128/aem.01998-08
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发表时间：
2009-02-15
影响因子：
4.4
通讯作者：
Lopez-Ferber, Miguel
中科院分区：
文献类型：
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作者：
Berling, Marie;Blachere-Lopez, Christine;Lopez-Ferber, Miguel
Cydia pomonella 颗粒病毒 (CpGV) 作为生物杀虫剂用于控制苹果蠹蛾 (Cydia pomonella) 已有 15 年的历史。 2004年，法国东南部首次发现了一些对该病毒低敏感性的昆虫种群。 RGV 是对田间使用的 CpGV-M 分离株具有抗性的苹果蠹蛾实验室群体，通过在田间收集抗性昆虫，然后将抗性性状渗入易感群体 (Sv) 中而建立。 RGV 菌落对 CpGV-M 分离株（欧洲所有商业病毒制剂中的活性成分）的耐药水平（基于 50% 致死浓度 [LC(50)s]）似乎是 Sv 菌落的 60,000 倍以上。在 RGV 上测试了来自其他各个地区的 CpGV 分离株的效率。其中，两种分离株（I12 和 NPP-R1）对 RGV 的致病性增强。在德国，I12 已被确定对抗性苹果蠹蛾菌落有效，并且观察到可以部分克服 RGV 菌落的抗性。最近鉴定的分离株 NPP-R1 对 RGV 的致病性比其他分离株更高，LC50 为 166 个包涵体 (OB)/μl，而 CpGV-M 的 LC50 为 1.36 x 10(6) OBs/μl。遗传表征表明，NPP-R1 是至少两种基因型的混合物，其中一种与 CpGV-M 相似。从 RGV 幼虫 NPP-R1 连续四代中获得的 2016-r4 分离株，其 CpGV-M 样基因型比例急剧降低，并且对 RGV 群体昆虫的致病性增加。
Cydia pomonella granulovirus (CpGV) has been used for 15 years as a bioinsecticide in codling moth (Cydia pomonella) control. In 2004, some insect populations with low susceptibility to the virus were detected for the first time in southeast France. RGV, a laboratory colony of codling moths resistant to the CpGV-M isolate used in the field, was established with collection of resistant insects in the field followed by an introgression of the resistant trait into a susceptible colony (Sv). The resistance level (based on the 50% lethal concentrations [LC(50)s]) of the RGV colony to the CpGV-M isolate, the active ingredient in all commercial virus formulations in Europe, appeared to be over 60,000-fold compared to the Sv colony. The efficiency of CpGV isolates from various other regions was tested on RGV. Among them, two isolates (I12 and NPP-R1) presented an increased pathogenicity on RGV. I12 had already been identified as effective against a resistant C. pomonella colony in Germany and was observed to partially overcome the resistance in the RGV colony. The recently identified isolate NPP-R1 showed an even higher pathogenicity on RGV than other isolates, with an LC50 of 166 occlusion bodies (OBs)/mu l, compared to 1.36 x 10(6) OBs/mu l for CpGV-M. Genetic characterization showed that NPP-R1 is a mixture of at least two genotypes, one of which is similar to CpGV-M. The 2016-r4 isolate obtained from four successive passages of NPP-R1 in RGV larvae had a sharply reduced proportion of the CpGV-M-like genotype and an increased pathogenicity against insects from the RGV colony.