PopP1, a new member of the YopJ/AvrRxv family of type III effector proteins, acts as a host-specificity factor and modulates aggressiveness of Ralstonia solanacearum
PopP1, a new member of the YopJ/AvrRxv family of type III effector proteins, acts as a host-specificity factor and modulates aggressiveness of Ralstonia solanacearum
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DOI：
10.1094/mpmi.2002.15.10.1058
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发表时间：
2002-10-01
影响因子：
3.5
通讯作者：
Boucher, C
中科院分区：
文献类型：
--
作者：
Lavie, M;Shillington, E;Boucher, C
植物病原细菌青枯菌基因组的一个11 kb长的区域的功能分析表明，它可能是通过水平基因转移获得的，该区域先前被确定为替代密码子使用区域（ACUR）。该ACUR编码一个插入序列和8个潜在的蛋白，其中一个与来自植物致病性欧文氏菌的宿主特异性因子部分同源，另一个是PopP 1，它与控制细菌与其宿主之间相互作用的III型分泌细菌效应子的YopJ/AvrRxv家族成员同源。除了ACUR中鉴定的一个基因外，对影响所有基因的突变体的分析表明，只有popP 1缺陷型菌株在植物感染试验中具有改变的表型。这种突变株对矮牵牛品系具有致病性，而矮牵牛品系对野生型菌株具有抗性。因此，popP 1是一个典型的无毒基因。我们表明，PopP 1蛋白的分泌，这种蛋白质的分泌需要一个功能III型分泌途径。与在R.在青枯菌中，popP 1基因的转录不与hrp基因的转录共调节，而是组成型的。
A functional analysis of an 11-kb-long region of the genome of the plant-pathogenic bacterium Ralstonia solana-cearum, previously identified as an alternative codon usage region (ACUR), reveals that it was probably acquired through horizontal gene transfer. This ACUR encodes an insertion sequence and eight potential proteins, one of which is partially homologous with a host-specificity factor from a plant-pathogenic Erwinia sp., and another, PopP1, which is homologous to members of the YopJ/AvrRxv family of type III-secreted bacterial effectors controlling interaction between bacteria and their hosts. The analysis of mutants affecting all except one of the genes identified in the ACUR showed that only the popP1-deficient strain had an altered phenotype in plant infection tests. This mutant strain became pathogenic to a Petunia line that is resistant to the wild-type strain. Therefore, popP1 behaves as a typical avirulence gene. We demonstrate that PopP1 protein is secreted and that secretion of this protein requires a functional type III-secretion pathway. In contrast to the structural genes for other type III-secreted proteins identified in R. solanacearum, transcription of the popP1 gene is not coregulated with transcription of hrp genes but is constitutive.