Comprehensive Identification of Principal Lipid Classes and Tocochromanols in Silkworm (Antheraea pernyi and Bombyx mori) Pupae Oils
Comprehensive Identification of Principal Lipid Classes and Tocochromanols in Silkworm (Antheraea pernyi and Bombyx mori) Pupae Oils
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蚕（柞蚕和家蚕）蛹油中主要脂质类和生育酚的综合鉴定
DOI：
10.1002/ejlt.201900280
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发表时间：
--
影响因子：
2.7
通讯作者：
Liao S.
中科院分区：
文献类型：
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作者：
Wang W.;Xu L.;Zou Y.;Pang D.;Shi W.;Mu L.;Li E.;Lan D.;Wang Y.;Liao S.
对柞蚕（A. pernyi）和家蚕（B. mori）的脂质成分和生育酚进行了综合鉴定。本研究报道了蚕蛹油。脂肪酸分析表明，两种油都含有高水平的不饱和脂肪酸（79.67% vs 71.11%），尤其是亚油酸和亚麻酸。此外，亚麻酸优先富集在三酰基甘油（TAGs）的第2位。液相色谱-质谱（LC - MS）分析表明，POO（含有一个棕榈酰和两个油酰的TAG）是主要的TAG形式，inA的百分比为20.18%。pernyi和15.00% inB。森。主要的磷脂种类是磷脂酰胆碱（PC， 30.40%比54.61%）和磷脂酰乙醇胺（PE， 34.82%比20.39%）。鉴定并定量分析了总含量分别为382.56±3.12和371.65±2.98µg g−1的4种甾醇成分。此外，测定了两种蚕蛹油中tocochromanol的含量（20.15±0.89 vs 17.15±0.71 mg g−1）。总的来说，蚕油是功能性脂质和生育酚的丰富来源。实际应用：本文系统地研究了柞蚕蛹和桑蚕油的主要脂类和桐香醇。这些数据为蚕油综合利用生产营养保健品提供了支持依据。
A comprehensive identification of lipid compositions and tocochromanols inAntheraea pernyi (A. pernyi) andBombyx mori(B. mori) pupae oil is reported in the present study. Fatty acid profiling shows that both oils contain high levels (79.67% vs 71.11%) of unsaturated fatty acids, especially linoleic acid and linolenic acid. Moreover, linolenic acid is preferentially enriched at thesn‐2 positions of triacylglycerols (TAGs). Liquid chromatograph‐mass spectrometry (LC‐MS) analysis demonstrates that POO (TAG with one palmitoyl and two oleoyls) is the primary TAG form with percentages of 20.18% inA. pernyi and 15.00% inB. mori. The dominating phospholipid species are phosphatidylcholine (PC, 30.40% vs 54.61%) and phosphatidylethanolamine (PE, 34.82% vs 20.39%). Four sterol constituents with total contents of 382.56 ± 3.12 and 371.65 ± 2.98 µg g−1are identified and analyzed quantitatively. Additionally, the levels of tocochromanols (20.15 ± 0.89 vs 17.15 ± 0.71 mg g−1) are quantified in both silkworm pupae oils. Overall, silkworm oil acts as an enriched source of functional lipids and tocochromanols.Practical Applications: A systematic investigation on the principal lipid classes and tocochromanols ofAntheraea pernyi pupae andBombyx moripupae oil is reported in this study. The informative data provide supporting evidence for comprehensive utilization of silkworm oil for production of nutritional and healthy products.