Programmed cell death in flight muscle histolysis of the house cricket.
Programmed cell death in flight muscle histolysis of the house cricket.
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DOI：
10.1016/j.jinsphys.2006.09.012
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发表时间：
2007
影响因子：
2.2
通讯作者：
R. H. Oliver;Acchia N J Albury;Timothy A Mousseau
中科院分区：
文献类型：
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作者：
R. H. Oliver;Acchia N J Albury;Timothy A Mousseau
我们的特点是飞行肌肉组织溶解的过程中，在女性的家蟋蟀，Acheta arcticus，通过分析的组织湿重，总蛋白质含量，和百分比缩短背纵向飞行肌肉（DLMs）的变化。我们的目的是（1）确定蟋蟀组织溶解的正常过程，（2）分析保幼激素（JH）去除和替代的影响，（3）确定放线菌酮治疗的影响，（4）检查组织溶解过程中蛋白质表达的模式。我们的研究结果表明，飞行肌肉组织溶解的房子蟋蟀是一个积极的，发育调节的细胞死亡程序诱导的内分泌信号的例子。DLM中总蛋白的初始下降表明诱导组织溶解的JH信号在第2天发生，并且组织溶解在第3天基本完成。在第3天蟋蟀的DLM中观察到组织重量和肌肉缩短百分比显著降低。第1天的蟋蟀颈部结扎阻止了组织溶解，但这种抑制作用可以通过用甲氧普烯（一种活性JH类似物）连续局部治疗来逆转，尽管第2天的蟋蟀结扎并不能阻止组织溶解。通过放线菌酮和35 S-甲硫氨酸的短期掺入的合成阻断分析证明了活性蛋白表达的需要。放线菌酮治疗防止组织溶解。通过电泳分离的DLM蛋白的自动荧光成像显示蛋白表达的明显协调调节。
We have characterized the process of flight muscle histolysis in the female house cricket, Acheta domesticus, through analysis of alterations of tissue wet weight, total protein content, and percent shortening of the dorsal longitudinal flight muscles (DLMs). Our objectives were to (1) define the normal course of histolysis in the cricket, (2) analyze the effects of juvenile hormone (JH) removal and replacement, (3) determine the effects of cycloheximide treatment, and (4) examine patterns of protein expression during histolysis. Our results suggest that flight muscle histolysis in the house cricket is an example of an active, developmentally regulated cell death program induced by an endocrine signal. Initial declines of total protein in DLMs indicated the JH signal that induced histolysis occurred by Day 2 and that histolysis was essentially complete by Day 3. Significant reductions in tissue weight and percent muscle shortening were observed in DLMs from Day 3 crickets. Cervical ligation of Day 1 crickets prevented histolysis but this inhibition could be reversed by continual topical treatments with methoprene (an active JH analog) although ligation of Day 2 crickets did not prevent histolysis. A requirement for active protein expression was demonstrated by analysis of synthesis block by cycloheximide and short-term incorporation of35S-methionine. Treatment with cycloheximide prevented histolysis. Autofluorographic imaging of DLM proteins separated by electrophoresis revealed apparent coordinated regulation of protein expression.