Surface conformational and linear epitopes on HPV-16 and HPV-18 L1 virus-like particles as defined by monoclonal antibodies
Surface conformational and linear epitopes on HPV-16 and HPV-18 L1 virus-like particles as defined by monoclonal antibodies
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DOI：
10.1006/viro.1996.0466
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发表时间：
1996-09-01
期刊：
影响因子：
3.7
通讯作者：
Galloway, DA
中科院分区：
文献类型：
--
作者：
Christensen, ND;Dillner, J;Galloway, DA
一组24个单克隆抗体（MAb）产生的人乳头瘤病毒（HPV）16型和18型L1病毒样颗粒（VLP）。筛选单克隆抗体对多种VLP的反应性，所述VLP由HPV-6、-11、-16、-18、-31、-33、-35和-45、棉尾兔乳头瘤病毒、牛乳头瘤病毒1型和一组跨越整个HPV-16 L1基因的35个重叠的20个氨基酸肽制备。检测到类型特异性线性和构象表面表位以及几个交叉反应线性表位，这些表位在不同生殖器HPV和动物乳头瘤病毒L1之间显示出不同水平的交叉反应性。大多数线性表位使用合成肽作图，并且表位被鉴定为表面或埋藏在VLP内，如通过使用完整和破坏的VLP抗原的ELISA中的反应性模式所定义的。这些单克隆抗体可能是有用的试剂，以帮助定义HPV-16和-18的中和表位时，感染性试验变得可用，并确定暴露在表面上或埋在组装的衣壳内的L1的区域。(C)出版社：Academic Press，Inc.
A panel of 24 monoclonal antibodies (MAbs) was generated against human papillomavirus (HPV) types 16 and 18 L1 virus-like particles (VLPs). The MAbs were screened for reactivity to a variety of VLPs prepared from HPV-6, -11, -16, -18, -31, -33, -35, and -45, cottontail rabbit papillomavirus, bovine papillomavirus type 1, and a set of 35 overlapping 20-amino-acid peptides spanning the entire HPV-16 L1 gene. Type-specific linear and conformational surface epitopes were detected as well as several cross-reactive linear epitopes that showed various levels of cross-reactivity between different genital HPV and animal papillomavirus L1s. Most of the linear epitopes were mapped using synthetic peptides, and the epitopes were identified as being either surface or buried within the VLP as defined by the pattern of reactivity in ELISA using intact and disrupted VLP antigen. These MAbs may be useful reagents to help define neutralizing epitopes of HPV-16 and -18 when infectivity assays become available, and to define the regions of L1 that are exposed on the surface or buried within the assembled capsid. (C) 1996 Academic Press, Inc.