Synergism Effect of the Essential Oil from Ocimum basilicum var. Maria Bonita and Its Major Components with Fluconazole and Its Influence on Ergosterol Biosynthesis.
Synergism Effect of the Essential Oil from Ocimum basilicum var. Maria Bonita and Its Major Components with Fluconazole and Its Influence on Ergosterol Biosynthesis.
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DOI：
10.1155/2016/5647182
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Alviano DS
中科院分区：
文献类型：
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作者：
Cardoso NN;Alviano CS;Blank AF;Romanos MT;Fonseca BB;Rozental S;Rodrigues IA;Alviano DS
摘要本研究旨在评价遗传改良品种basilicum var. Maria Bonita的EO及其主要成分对氟康唑敏感和耐药菌株白色念珠菌和新型隐球菌的活性。香叶醇具有较低的MIC（对新生假丝酵母菌的MIC为76 μg/mL，对两种假丝酵母菌的MIC均为152 μg/mL）。EO、芳樟醇和香叶醇与氟康唑联合使用可增强其抗真菌活性，特别是对耐药菌株（MIC分别降至156、197和38 μg/mL）。麦角甾醇测定表明，亚抑制浓度的物质能够减少甾醇的提取量。所测试的物质能够减小胶囊大小，这表明它们具有重要的作用机制。透射电镜显示，亚抑制浓度处理后，新生C.细胞壁被破坏。白色念珠菌的超微结构改变，如膜不规则、囊泡的存在和细胞壁增厚。两种白色念珠菌在MIC和2次MIC下的生物膜形成均受到抑制。这些结果进一步支持了basilicum EO及其主要成分作为抗真菌药物的潜在来源。
The aim of this study was to evaluate the activity of the EO and its major components of Ocimum basilicum var. Maria Bonita, a genetically improved cultivar, against the fluconazole sensitive and resistant strains of Candida albicans and Cryptococcus neoformans. Geraniol presented better results than the EO, with a low MIC (76 μg/mL against C. neoformans and 152 μg/mL against both Candida strains). The combination of EO, linalool, or geraniol with fluconazole enhanced their antifungal activity, especially against the resistant strain (MIC reduced to 156, 197, and 38 μg/mL, resp.). The ergosterol assay showed that subinhibitory concentrations of the substances were able to reduce the amount of sterol extracted. The substances tested were able to reduce the capsule size which suggests they have an important mechanism of action. Transmission electron microscopy demonstrated cell wall destruction of C. neoformans after treatment with subinhibitory concentrations. In C. albicans ultrastructure alterations such as irregularities in the membrane, presence of vesicles, and cell wall thickening were observed. The biofilm formation was inhibited in both C. albicans strains at MIC and twice MIC. These results provide further support for the use of O. basilicum EO and its major components as a potential source of antifungal agents.