Insights into spawning behavior and development of the European amphioxus (Branchiostoma lanceolatum)
Insights into spawning behavior and development of the European amphioxus (Branchiostoma lanceolatum)
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DOI：
10.1002/jez.b.21179
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发表时间：
2007-07-15
影响因子：
2.2
通讯作者：
Escriva, Hector
中科院分区：
文献类型：
--
作者：
Fuentes, Michael;Benito, Elia;Escriva, Hector
头索动物文昌鱼（Branchiostoma sp.）是研究脊索动物发育机制进化的重要动物模型。获得文昌鱼胚胎是这些研究的关键步骤。研究表明，水温升高3-4摄氏度会触发欧洲文昌鱼（Branchiostoma lanceolatum）在人工饲养条件下产卵，然而，人们对该物种在野外的自然产卵行为知之甚少。在这项工作中，我们遵循了产卵行为的欧洲文昌鱼在两个产卵季节（2004年和2005年），无论是在外地和圈养。我们发现，在该领域的动物产卵大约从5月中旬到7月初，但根据一年，他们表现出不同的模式产卵。因此，即使温度在人工饲养的产卵诱导中起关键作用，但它不是该领域的主要因素。此外，我们报告了一些改进的方法，诱导产卵在圈养（例如，在维护，光周期控制和诱导产卵在实验室没有运行的海水系统）。这些研究对文昌鱼养殖和改进实验室技术以开发文昌鱼作为实验动物模型具有重要意义。
The cephalochordate amphioxus (Branchiostoma sp.) is an important animal model for studying the evolution of chordate developmental mechanisms. Obtaining amphioxus embryos is a key step for these studies. It has been shown that an increase of 3-4 degrees C in water temperature triggers spawning of the European amphioxus (Branchiostoma lanceolatum) in captivity, however, very little is known about the natural spawning behavior of this species in the field. In this work, we have followed the spawning behavior of the European amphioxus during two spawning seasons (2004 and 2005), both in the field and in captivity. We show that animals in the field spawn approximately from mid-May through early July, but depending on the year, they show different patterns of spawning. Thus, even if temperature has a critical role in the induction of the spawning in captivity, it is not the major factor in the field. Moreover, we report some improvements on the methodology for inducing spawning in captivity (e.g. in maintenance, light cycle control and induction of spawning in a laboratory without running sea water system). These studies have important implications for amphioxus animal husbandry and for improving laboratory techniques to develop amphioxus as an experimental animal model.