Expression and localization of Bombyx mori nucleopolyhedrovirus GP37
Expression and localization of Bombyx mori nucleopolyhedrovirus GP37
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DOI：
10.1007/s11262-023-01983-3
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发表时间：
2023-03
期刊：
影响因子：
1.6
通讯作者：
S. Fujimoto;Kaito Fujimaki;Tomohiro Suzuki;S. Katsuma;M. Iwanaga
中科院分区：
文献类型：
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作者：
S. Fujimoto;Kaito Fujimaki;Tomohiro Suzuki;S. Katsuma;M. Iwanaga
线粒体在细胞内能量代谢中起重要作用。本研究描述了家蚕核多角体病毒（BmNPV） GP37 （BmGP37）在宿主线粒体中的作用。本研究通过双向凝胶电泳技术，比较了从bmnpv感染细胞和模拟感染细胞中分离到的宿主线粒体相关蛋白。通过液相色谱-质谱分析，鉴定出病毒感染细胞中一个线粒体相关蛋白BmGP37。在bmnpv感染的BmN细胞中，生成了与BmGP37特异性反应的BmGP37抗体。Western blot实验表明，BmGP37在感染后18 h表达，证实其为线粒体相关蛋白。免疫荧光分析表明，BmGP37在BmNPV感染过程中定位于宿主线粒体。此外，western blot分析显示BmGP37是BmNPV闭塞衍生病毒（ODV）的一个新的组分蛋白。上述结果表明BmGP37是odv相关蛋白之一，在BmNPV感染过程中可能在宿主线粒体中发挥重要作用。
Mitochondria play an essential role in intracellular energy metabolism. This study described the involvement of Bombyx mori nucleopolyhedrovirus (BmNPV) GP37 (BmGP37) in host mitochondria. Herein, the proteins associated with host mitochondria isolated from BmNPV-infected or mock-infected cells by two-dimensional gel electrophoresis were compared. One mitochondria-associated protein in virus-infected cells was identified as BmGP37 by liquid chromatography-mass spectrometry analysis. Furthermore, the BmGP37 antibodies were generated, which could react specifically with BmGP37 in the BmNPV-infected BmN cells. Western blot experiments showed that BmGP37 was expressed at 18 h post-infection and was verified as a mitochondria-associated protein. Immunofluorescence analysis demonstrated that BmGP37 localized to the host mitochondria during BmNPV infection. Furthermore, western blot analysis revealed that BmGP37 is a novel component protein of the occlusion-derived virus (ODV) of BmNPV. The present results indicated that BmGP37 is one of the ODV-associated proteins and may have important roles in host mitochondria during BmNPV infection.