The Lipophilic Vitamin C Derivative, 6-O-Palmitoylascorbate Protects Human Keratinocytes and 3D-Human Skin Equivalents Against X-Ray-Induced Oxidative Stress and Apoptosis More Markedly Than L-Ascorbic Acid.
The Lipophilic Vitamin C Derivative, 6-O-Palmitoylascorbate Protects Human Keratinocytes and 3D-Human Skin Equivalents Against X-Ray-Induced Oxidative Stress and Apoptosis More Markedly Than L-Ascorbic Acid.
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亲脂性维生素 C 衍生物 6-O-棕榈酰抗坏血酸比 L-抗坏血酸更能显着保护人类角质形成细胞和 3D 人类皮肤等效物免受 X 射线诱导的氧化应激和细胞凋亡。
DOI：
10.1002/jcb.25639
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Miwa N.
中科院分区：
文献类型：
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作者：
Xiao L;Miwa N.
本研究的目的是研究亲脂性维生素C衍生物6‐O‐棕榈酰抗坏血酸酯（PlmtVC）对X射线辐射诱导的有害事件的预防作用。自由基清除能力测试表明，在相同条件下，PlmtVC的新鲜溶液和陈化溶液（37℃保存72 h）对DPPH和过氧自由基（ROO·）的清除能力都明显高于L‐抗坏血酸（L‐AA），这表明PlmtVC是一种比L‐AA更有效和稳定的抗氧化剂。X射线（15 Gy）照射增加了人角化细胞HaCaT细胞内ROS的产生、脂质过氧化和蛋白质羰基化，所有这些都被PlmtVC比L‐AA更明显地抑制，尤其是细胞内ROS。X射线（15gy）照射后，HaCaT细胞或3D皮肤组织中caspase 3/7激活和TUNEL检测到的DNA链断裂特征的凋亡分别明显增加，PlmtVC比L‐AA更明显地阻止了这两种情况。PlmtVC还显著阻止异丙苯氢过氧化氢诱导的3D皮肤表皮部分细胞ROS的产生。因此，PlmtVC通过其抗氧化活性和棕榈酰基亲脂性，比L - AA更有效地预防X射线诱导的各种有害影响。j .细胞。中国生物医学工程学报，2017,31(2):379 - 379。©2016 Wiley期刊公司
The aim of this study was to investigate preventive effects of the lipophilic vitamin C derivative, 6‐O‐palmitoylascorbate (PlmtVC) against X‐ray radiation‐induced harmful events. Free radical scavenging activity tests showed that both fresh and old (being kept at 37°C for 72 h) solutions of PlmtVC showed significantly higher abilities for scavenging both DPPH and peroxyl radical (ROO·) radicals than L‐ascorbic acid (L‐AA) under the same conditions, suggesting that PlmtVC is an antioxidant more efficient and stable than L‐AA. Irradiation with X‐ray (15 Gy) increased intracellular ROS production, lipid peroxidation and protein carbonylation, in human keratinocytes HaCaT, all of which were repressed, especially for intracellular ROS more markedly, by PlmtVC than by L‐AA. After X‐ray (15 Gy)‐irradiation, caspase 3/7 activation and TUNEL‐detected DNA‐strand‐breakages characteristic of apoptosis obviously increased in HaCaT cells or 3D‐skin tissue equivalents, respectively, both of which were prevented more appreciably by PlmtVC than by L‐AA. PlmtVC also noticeably prevented cumene hydroperoxide‐induced generation of cellular ROS in epidermis parts of 3D‐skin equivalents. Thus, PlmtVC prevents X‐ray‐induced diverse harmful effects, through its antioxidant activity and the palmitoyl moiety‐based lipophilicity, more efficiently than L‐AA. J. Cell. Biochem. 118: 318–329, 2017. © 2016 Wiley Periodicals, Inc.