Structure and developmentally regulated expression of a Strongylocentrotus purpuratus collagen gene.
Structure and developmentally regulated expression of a Strongylocentrotus purpuratus collagen gene.
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Strongylocentrotus purpuratus 胶原蛋白基因的结构和发育调控表达。
DOI：
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发表时间：
1986
影响因子：
11.1
通讯作者：
R. Simpson
中科院分区：
文献类型：
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作者：
M. Venkatesan;F. de Pablo;G. Vogeli;R. Simpson
我们用小鼠α1(IV型)胶原基因探针分离并部分鉴定了一个约20kb碱基对的紫球网眼线虫基因组克隆。克隆的一个1千碱基对的HindIII片段与探针强烈杂交；这已被亚克隆和测序。它包含212个碱基对的序列，编码(Gly-Xaa-Yaa)n(其中xaa和Yaa是不同的未指定氨基酸)，这是所有已知胶原基因的特征。在这个外显子的重复模式中有一个单点不连续，类似于小鼠IV型胶原的基因组结构。(Gly-Xaa-Yaa)n-编码元件两侧是共有剪接序列，其两侧的插入序列具有多个同相终止密码子。在电子显微镜下，噬菌体Lambda重组克隆和Poly(A)+RNA之间的R环显示了多个短外显子，这一特征也见于脊椎动物的胶原基因。(Gly-Xaa-Yaa)n蛋白编码序列与发育调节的9kb碱基的mRNA杂交；该信息出现在桑椹胚期，在囊胚期丰度急剧上升，并在发育后期与总RNA的比例下降。
We have isolated and partially characterized an ca. 20-kilobase-pair Strongylocentrotus purpuratus genomic clone, using a mouse alpha 1 (type IV) collagen cDNA probe. A 1-kilobase-pair HindIII fragment of the clone hybridizes strongly to the probe; this has been subcloned and sequenced. It contains 212 base pairs of sequence coding for (Gly-Xaa-Yaa)n (where Xaa and Yaa are different unspecified amino acids), characteristic of all known collagen genes. There is a single point of discontinuity within the repeating pattern in this exon, similar to the genomic structure of mouse type IV collagen. The (Gly-Xaa-Yaa)n-encoding element is flanked by consensus splicing sequences, and the intervening sequences on either side of it have multiple in-phase termination codons. Electron microscopy of R loops between the phage lambda recombinant clone and poly(A)+ RNA reveals multiple short exons, a feature also seen in vertebrate collagen genes. The (Gly-Xaa-Yaa)n protein-encoding sequence hybridizes to a developmentally regulated 9-kilobase mRNA; the message appears during the morula stage, rises sharply in abundance at the blastula stage, and decreases in proportion to total RNA later in development.