A RAPID SENSITIVE SILVER STAIN FOR POLYPEPTIDES IN POLYACRYLAMIDE GELS
A RAPID SENSITIVE SILVER STAIN FOR POLYPEPTIDES IN POLYACRYLAMIDE GELS
复制标题
DOI：
10.1016/0003-2697(81)90136-6
复制
发表时间：
1981-01-01
影响因子：
2.9
通讯作者：
GOLDMAN, D
中科院分区：
文献类型：
--
作者：
MERRIL, CR;DUNAU, ML;GOLDMAN, D
使用银来检测多肽最初是通过修改组织染色实现的。通过采用光化学方法，发展了一种新的多肽银染色法，它几乎同样灵敏，但比以前的方法效率高得多。新的银染色只使用3种溶液，并允许在50分钟内观察到蛋白质模式。它的敏感度是考马斯蓝的100倍。
The use of silver to detect polypeptides was originally achieved by modifying tissue stains. By adapting methods of photochemistry a new Ag stain was developed for polypeptides which is nearly as sensitive but much more efficient than these earlier procedures. The new Ag stain utilizes only 3 solutions and allows protein patterns to be visualized within 50 min. Its sensitivity is 100 times that of the Coomassie blue stain.