Late radiation response of kidney assayed by tubule-cell survival.
Late radiation response of kidney assayed by tubule-cell survival.
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DOI：
10.1259/0007-1285-59-702-587
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发表时间：
1986-06
期刊：
影响因子：
--
通讯作者：
H. Withers;K. Mason;H. Thames
中科院分区：
文献类型：
--
作者：
H. Withers;K. Mason;H. Thames
建立了小鼠肾小管细胞存活测定方法。它类似于其他原位克隆细胞存活测定。用137Cs照射器照射一个肾脏，60-68周后切除进行组织学检查。在未受辐射的动物中，在肾脏中部冠状横切面上约有370个小管与胶囊接触。照射后，广泛的小管损伤是主要病变。与胶囊接触的上皮化小管数量呈剂量依赖性对数递减。在11-16 Gy的剂量范围内，D0值为1.5 Gy，描述了负责小管上皮再生的克隆原细胞的剂量-存活关系。这种放射敏感性类似于急性反应组织中的干细胞。在小管最初剥离上皮时动脉血管损伤的组织学证据的缺乏，以及肾小管细胞与其他哺乳动物细胞放射敏感性的相似性，支持了“晚期”辐射损伤主要由实质细胞耗损引起的假设，而不是间接由血管损伤引起的，这是普遍认为的。
An assay for the survival of renal tubule cells was developed using mice. It is analogous to other in-situ clonogenic cell survival assays. One kidney was irradiated using a 137Cs irradiator and removed 60-68 weeks later for histological examination. In unirradiated animals there were about 370 tubules in contact with the capsule in a coronal cross section at the middle of the kidney. After irradiation, extensive tubular damage was the dominant lesion. The number of epithelialized tubules in contact with the capsule showed a dose-dependent logarithmic decline. The dose-survival relationship for the clonogenic cells responsible for the regeneration of tubule epithelium was described by a D0 value of 1.5 Gy over the dose range 11-16 Gy. This radiosensitivity resembles that of stem cells in acutely responding tissues. The lack of histological evidence of damage to the arterial vasculature at the time the tubules are initially denuded of epithelium, and the similarity of renal tubule cell radiosensitivity to that of other mammalian cells, support the hypothesis that "late" radiation injury results primarily from depletion of parenchymal cells, not indirectly from injury to blood vessels, as has been the prevailing belief.