Functional replacement of Drosophila Btk29A with human Btk in male genital development and survival
Functional replacement of Drosophila Btk29A with human Btk in male genital development and survival
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DOI：
10.1016/j.febslet.2005.06.042
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发表时间：
2005-08-01
期刊：
影响因子：
3.5
通讯作者：
Yamamoto, D
中科院分区：
文献类型：
--
作者：
Hamada, N;Bäckesjö, CM;Yamamoto, D
果蝇2型Btk 29 A揭示了哺乳动物Tee激酶中与Btk的最高同源性。在Btk 29 A（ficP）突变雄性中，支撑阴茎的apodeme分成两部分。人类Btk在39%的Btk 29，4A（ficP）雄性中挽救了这种表型，而果蝇转基因在90-100%的突变体中做到了这一点。Btk 29 A（ficP）突变使成年寿命降低至野生型的11%。这种效应被果蝇2型抵消，产生了野生型寿命的76%。人类Btk将Btk 29 A（ficP）突变体的寿命延长至野生型的20%。因此，人类Btk可以部分取代果蝇Btk 29 A（+）在男性生殖器发育和生存。(c)2005年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
Drosophila type 2 Btk29A reveals the highest homology to Btk among mammalian Tee kinases. In Btk29A(ficP) mutant males, the apodeme holding the penis split into two pieces. Human Btk rescued this phenotype in 39% of Btk29,4A(ficP) males, while the Drosophila transgenes did so in 90-100% of mutants. The Btk29A(ficP) mutation reduced adult longevity to 11% that of wild-type. This effect was counteracted by Drosophila type 2, yielding 76% of the wild-type lifespan. Human Btk extended the lifespan of Btk29A(ficP) mutants only to 20% that of wild-type. Thus human Btk can partially replace Drosophild Btk29A(+) in male genital development and survival. (c) 2005 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.