Supramolecular crafting of cell adhesion
Supramolecular crafting of cell adhesion
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DOI：
10.1016/j.biomaterials.2007.06.026
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发表时间：
2007-11-01
期刊：
影响因子：
14
通讯作者：
Stupp, Samuel I.
中科院分区：
文献类型：
--
作者：
Storrie, Hannah;Guler, Mustafa O.;Stupp, Samuel I.
超分子设计具有生物活性的人工细胞外基质以控制细胞行为在细胞治疗和细胞测定中至关重要。在这一领域的大多数以前的工作集中在聚合物或单分子膜，排除控制信号密度和可访问性的纳米级丝状环境中的天然基质。我们在这里使用了自组装超分子纳米纤维，其将细胞粘附配体RGDS以货车德瓦耳斯密度展示给细胞。在这种非常高的密度下的信号可及性已经通过分子结构的变化而变化，因此通过形成纤维的单体的超分子包装而变化。我们发现，单体的分支结构和随之而来的较低的包装效率和表位运动的额外空间改善了AM信号传导，用于细胞粘附、扩散和迁移。使用具有极高表位密度的纳米级物体的人工基质可以促进用于信号传导的受体聚集，并且还使基质和细胞之间的移动的三维界面处的配体和受体之间的成功结合最大化。人工生物活性细胞外基质的超分子设计来调节细胞反应可能被证明是再生医学和研究生物过程的有力策略。(c)2007爱思唯尔有限公司保留所有权利。
The supramolecular design of bioactive artificial extracellular matrices to control cell behavior is of critical importance in cell therapies and cell assays. Most previous work in this area has focused on polymers or monolayers which preclude control of signal density and accessibility in the nanoscale filamentous environment of natural matrices. We have used here self-assembling supramolecular nanofibers that display the cell adhesion ligand RGDS at van der Waals density to cells. Signal accessibility at this very high density has been varied by changes in molecular architecture and therefore through the supramolecular packing of monomers that form the fibers. We found that branched architectures of the monomers and the consequent lower packing efficiency and additional space for epitope motion improves AM signaling for cell adhesion, spreading, and migration. The use of artificial matrices with nanoscale objects with extremely high epitope densities could facilitate receptor clustering for signaling and also maximize successful binding between ligands and receptors at mobile three-dimensional interfaces between matrices and cells. Supramolecular design of artificial bioactive extracellular matrices to tune cell response may prove to be a powerful strategy in regenerative medicine and to study biological processes. (c) 2007 Elsevier Ltd. All rights reserved.