Molecular cloning and characterization of common marmoset SULT1C subfamily members that catalyze the sulfation of thyroid hormones
Molecular cloning and characterization of common marmoset SULT1C subfamily members that catalyze the sulfation of thyroid hormones
复制标题
催化甲状腺激素硫酸化的普通狨猴 SULT1C 亚家族成员的分子克隆和表征
DOI：
10.1093/bbb/zbab141
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Sakakibara Yoichi
中科院分区：
文献类型：
--
作者：
Kurogi Katsuhisa;Manabe Yoko;Liu Ming-Cheh;Suiko Masahito;Sakakibara Yoichi
细胞溶质磺基转移酶SULT 1C亚家族是哺乳动物进化过程中最灵活的基因亚家族之一。SULT 1C酶的生理功能仍有待充分了解。在这项研究中，普通绒猴（Callithrix jacchus），一个有前途的灵长类动物模型，被用来调查的SULT 1C亚家族的功能相关性。基因数据库检索发现3个完整的SULT 1C基因和1个假基因。根据序列同源性和基因位置，这4个基因被命名为SULT 1C 1、SULT 1C 2、SULT 1C 3 P和SULT 1C 5。由于SULT 1C 5是人SULT 1C 2 P的直向同源基因，因此我们建议重新将人SULT 1C 2 P命名为SULT 1C 5 P。纯化的重组SULT 1C酶对各种异生物质化合物和甲状腺激素的硫酸化活性。动力学分析表明，SULT 1C 1和SULT 1C 5对3，3 ′-T2具有较高的催化活性。因此，SULT 1C亚型可能在普通绒猴的甲状腺激素代谢中发挥作用。
Cytosolic sulfotransferase SULT1C subfamily is one of the most flexible gene subfamilies during mammalian evolution. The physiological functions of SULT1C enzymes still remain to be fully understood. In this study, common marmoset (Callithrix jacchus), a promising primate animal model, was used to investigate the functional relevance of the SULT1C subfamily. Gene database search revealed 3 intactSULT1Cgenes and a pseudogene in its genome. These 4 genes were namedSULT1C1, SULT1C2, SULT1C3P, andSULT1C5, according to the sequence homology and gene location. SinceSULT1C5is the orthologous gene for humanSULT1C2P, we propose, here, to revisit the designation of humanSULT1C2Pto SULT1C5P. Purified recombinant SULT1C enzymes showed sulfating activities toward a variety of xenobiotic compounds and thyroid hormones. Kinetic analysis revealed high catalytic activities of SULT1C1 and SULT1C5 for 3,3′-T2. It appears therefore that SULT1C isoforms may play a role in the thyroid hormone metabolism in common marmoset.