Qualitative and quantitative contrast-enhanced ultrasonographic assessment of cerulein-induced acute pancreatitis in dogs.
Qualitative and quantitative contrast-enhanced ultrasonographic assessment of cerulein-induced acute pancreatitis in dogs.
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DOI：
10.1111/jvim.12319
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发表时间：
2014-03
影响因子：
2.6
通讯作者：
Takiguchi M
中科院分区：
文献类型：
--
作者：
Lim SY;Nakamura K;Morishita K;Sasaki N;Murakami M;Osuga T;Ohta H;Yamasaki M;Takiguchi M
急性胰腺炎（AP）是犬外分泌胰腺最常见的疾病，准确的无创诊断具有挑战性。目的：探讨应用定量超声造影（CEUS）检测猪蓝蛋白诱导AP胰腺灌注变化的可行性。六只成年雌性比格犬。每只狗静脉注射7.5 μg/kg/h生理盐水稀释的毛蛋白2小时。作为对照，所有犬在注射蓝蛋白前2周静脉滴注生理盐水2小时。分别于注射生理盐水和蓝蛋白后0小时、2小时、4小时、6小时和12小时行胰腺和十二指肠超声造影。时间-强度曲线从胰腺和十二指肠感兴趣的区域创建。测量5个灌注参数进行统计分析：初始上斜时间、峰值时间、冲洗时间、峰值强度（PI）和曲线下面积（AUC）。在cerulein诱导的AP中，胰腺PI在2小时和4小时与0小时相比增加，在2小时、4小时和6小时与对照组相比增加。与0小时相比，4小时时AUC增加，与对照组相比，2和4小时时AUC增加。与对照组相比，洗出时间延长了4小时。生理盐水对照，2小时的峰值时间比0小时更快。超声造影参数PI和AUC可为鉴别急性胰腺炎和正常胰腺提供有用的信息。蓝蛋白诱导的AP在超声造影上表现为长时间的高回声增强。
Acute pancreatitis (AP) is the most common disease of the canine exocrine pancreas, and accurate noninvasive diagnosis is challenging. To determine the feasibility of using quantitative contrast‐enhanced ultrasonography (CEUS) to detect pancreatic perfusional changes in cerulein‐induced AP in dogs. Six adult female Beagles. Each dog received 2 hours of IV infusion with 7.5 μg/kg/h of cerulein diluted in saline. As control, all dogs received 2 hours of IV infusion of saline 2 weeks before cerulein infusion. CEUS of the pancreas and duodenum were performed before (0 hour), and at 2, 4, 6, and 12 hours after saline and cerulein infusion. Time‐intensity curves were created from regions of interest in the pancreas and duodenum. Five perfusional parameters were measured for statistical analysis: time to initial up‐slope, peak time, time to wash‐out, peak intensity (PI), and area under the curve (AUC). In cerulein‐induced AP, pancreatic PI increased at 2 and 4 hours when compared to 0 hour, and at 2, 4, and 6 hours when compared to control. AUC increased at 4 hours when compared to 0 hour, and at 2 and 4 hours when compared to control. Time to wash‐out was prolonged at 4 hours when compared to control. For saline control, peak time was faster at 2 hours when compared to 0 hour. CEUS parameters PI and AUC can provide useful information in differentiating acute pancreatitis from normal pancreas. Cerulein‐induced AP was characterized by prolonged hyperechoic enhancement on CEUS.