EFFECTS OF NO SYNTHASE INHIBITION ON THE MUSCULAR BLOOD-FLOW RESPONSE TO TREADMILL EXERCISE IN RATS
EFFECTS OF NO SYNTHASE INHIBITION ON THE MUSCULAR BLOOD-FLOW RESPONSE TO TREADMILL EXERCISE IN RATS
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DOI：
10.1152/jappl.1994.77.3.1288
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发表时间：
1994-09-01
影响因子：
3.3
通讯作者：
MUSCH, TI
中科院分区：
文献类型：
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作者：
HIRAI, T;VISNESKI, MD;MUSCH, TI
在清醒的雄性Sprague-Dawley大鼠（603 +/- 28 g; n = 6）中研究了一氧化氮（NO）释放在调节运动骨骼肌的血流量（BF）中的功能作用。在这项研究中，BF使用放射性标记的微球测量在跑台运动（10%等级，20 m/min）之前和之后的NO合成酶（NOS）抑制剂N-G-硝基-L-精氨酸甲酯（30 mg/kg ia）。NOS抑制后，平均动脉血压从静息基线值升高，注射乙酰胆碱（ACh）13.0和10.0 μ g/kg（ia）后血管舒张反应的持续时间缩短（P < 0.05），表明NOS功能降低。在运动过程中，NOS抑制后，肾脏和肠道器官的BF减少。此外，在28个单独的后躯肌肉或肌肉部位中，有16个的BF减少。此外，BF的这些减少与每块肌肉中发现的快收缩氧化糖酵解（FOG）和慢收缩氧化（SO）型纤维的百分比的估计总和线性相关[Δ BF = -1.1（%SO + %FOG）+ 16.4; r = 0.88，P < 0.001]。这些结果表明，NO介导的血管舒张有助于BF反应内和运动过程中的大鼠后躯的肌肉之间。
The functional role of nitric oxide (NO) release in regulating blood flow (BF) to exercising skeletal muscle was studied in conscious male Sprague-Dawley rats (603 +/- 28 g; n = 6). In this study, BF was measured using radiolabeled microspheres during treadmill exercise (10% grade, 20 m/min) before and after NO synthase (NOS) inhibition with N-G-nitro-L-arginine methyl ester (30 mg/kg ia). After NOS inhibition, mean arterial blood pressure increased from resting baseline values and the duration of vasodilator responses to acetylcholine (ACh) injections 13.0 and 10.0 mu g/kg ia) was diminished (P < 0.05), demonstrating reduced NOS function. During exercise, BF to the kidneys and organs of the gut was reduced after NOS inhibition. In addition, BF was reduced in 16 of the 28 individual hindquarter muscles or muscle parts. Moreover these reductions in BF were linearly correlated with the estimated sum of the percentage of fast-twitch oxidative glycolytic (FOG) and slow-twitch oxidative (SO) types of fibers found in each muscle [Delta BF = -1.1 (%SO + %FOG) + 16.4; r = 0.88, P < 0.001]. These results suggest that NO-mediated vasodilation contributes to the BF responses within and among the muscles of the rat's hindquarters during exercise.