Vertical distribution of metabolically active eukaryotes in the water column and sediments of the Black Sea
Vertical distribution of metabolically active eukaryotes in the water column and sediments of the Black Sea
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DOI：
10.1111/j.1574-6941.2009.00756.x
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发表时间：
2009-12-01
影响因子：
4.2
通讯作者：
Shtereva, Galina
中科院分区：
文献类型：
--
作者：
Coolen, Marco J. L.;Shtereva, Galina
最近的基于DNA的系统发育研究报告了高真核生物在广泛的设置，包括从缺氧环境中获得的样品中的二聚体。然而，需要进行基于RNA的平行调查，以核实所检测到的物种在这种极端环境中是否确实具有代谢活性。黑海是世界上最大的缺氧盆地，但在分子真核生物多样性研究方面仍然采样不足。在这里，我们报告的分布活跃的真核生物（18S rRNA为基础的调查）沿着垂直营养和氧化还原梯度的水柱和黑海的表层沉积物。各种各样的真核生物都活跃在低氧的深水沃茨中。值得注意的是，某些物种是活跃的，但在平行的18S rDNA调查中逃脱了检测。表层沉积物的18S rDNA调查产生的浮游起源的类群，但没有这些被确定为在采样时的水柱。我们的数据还表明，基因转录并不总是提供明确的证据表明，活跃的微生物是土著的一个特定的位置在环境梯度，因为某些动物园和浮游植物物种仍然是可行的，可检测到18 S rRNA在长达300年的硫化物沉积物，下面类似于830在硫化物沃茨。
Recent DNA-based phylogenetic studies have reported high eukaryotal diversities in a wide range of settings including samples obtained from anoxic environments. However, parallel RNA-based surveys are required in order to verify whether the species detected are in fact metabolically active in such extreme environments. The Black Sea is the World's largest anoxic basin but remains undersampled with respect to molecular eukaryotic diversity studies. Here, we report the distribution of active eukaryotes (18S rRNA-based survey) along a vertical nutrient and redox gradient in the water column and surface sediments of the Black Sea. A wide variety of eukaryotes were active in suboxic deep waters. Notably, certain species were active but escaped detection during a parallel 18S rDNA survey. The 18S rDNA survey from surface sediments yielded taxa of pelagic origin but none of these were identified from the water column at the time of sampling. Our data also indicate that gene transcripts do not always provide unequivocal proof that active microorganisms are indigenous to a specific position in an environmental gradient, because certain zoo- and phytoplankton species were still viable with detectable 18S rRNA in up to 300-year-old sulfidic sediments that underlie similar to 830 in of sulfidic waters.