Expression of EGFL7 in primordial germ cells and in adult ovaries and testes
Expression of EGFL7 in primordial germ cells and in adult ovaries and testes
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DOI：
10.1016/j.gep.2008.05.001
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发表时间：
2008-07-01
影响因子：
1.2
通讯作者：
Stuhmann, Heidi
中科院分区：
文献类型：
--
作者：
Campagnolo, Luisa;Moscatelli, Ana;Stuhmann, Heidi
我们以前已经报道了一种新的内皮限制性基因Egfl7的分离和表征，该基因编码约30 kDa的分泌蛋白。我们和其他人证明了Egfl7在胚胎发育过程中由内皮细胞高度表达，并在成人血管系统中下调。在本文中，我们表明，在小鼠胚胎发育过程中，Egfl7也表达的原始生殖细胞（PGC）。当PGCs分化为前精原细胞和卵原细胞时，表达下调，并且到15.5dpc，在两种性别的生殖系中不再检测到Egfl7。值得注意的是，Egfl7在成年睾丸的生殖细胞中再次瞬时上调。相反，卵巢中的表达仍然限于血管内皮。我们的研究结果提供了EGFL7非内皮表达的第一个证据，并表明EGFL7在血管发育和生殖细胞分化中的独特作用。(C)2008 Elsevier B.V.保留所有权利。
We have previously reported the isolation and characterization of a novel endothelial-restricted gene, Egfl7, that encodes a secreted protein of about 30-kDa. We and others demonstrated that Egfl7 is highly expressed by endothelial cells during embryonic development and becomes down-regulated in the adult vasculature. In the present paper, we show that during mouse embryonic development, Egfl7 is also expressed by primordial germ cells (PGC). Expression is down-regulated when PGCs differentiate into pro-spermatogonia and oogonia, and by 15.5 dpc Egfl7 can no longer be detected in the germ line of both sexes. Notably, Egfl7 is again transiently up-regulated in germ cells of the adult testis. In contrast, expression in the ovary remains limited to the vascular endothelium. Our results provide the first evidence of a non-endothelial expression of EGFL7 and suggest distinctive roles for Egfl7 in vascular development and germ cell differentiation. (C) 2008 Elsevier B.V. All rights reserved.