Identification and characterization of the Anillin Gene in silkworm
Identification and characterization of the Anillin Gene in silkworm
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家蚕 Anillin 基因的鉴定与表征
DOI：
10.1089/dna.2019.4660
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Daojun Cheng
中科院分区：
文献类型：
--
作者：
Peng Wang;Wenliang Qian;Weina Wang;Mengpei Guo;Qingyou Xia;Daojun Cheng
苯胺素是一种肌动蛋白结合蛋白，在后生动物有丝分裂细胞周期进程中起着至关重要的作用。然而，在家蚕（Bombyx mori）中，anillingene的序列和功能尚未被确定。在本研究中，我们克隆了家蚕青霉素（BmAnillin）基因的全长cDNA序列。BmAnillin蛋白的氨基酸序列包括一个覆盖Anillin同源结构域和一个pleckstrin同源结构域的Anillin同源区（AHR）。家蚕和其他生物的anillingenes的系统发育分析和多重比对表明，含有保守磷酸化位点的AHR具有进化保守性。逆转录- pcr实验证实，bmanillingene在有丝分裂周期的性腺幼虫发育过程中高表达，在幼虫蜕皮过程中具有内循环的丝腺中意外高表达。在家蚕卵巢BmN4-SID1细胞中，RNA干扰介导的bmanillingene的下调破坏了染色体分离，导致后期卵裂沟处f -肌动蛋白丝的丢失，表明bmanillingene在家蚕细胞分裂中是必不可少的。
Anillin is an actin binding protein and plays crucial roles during mitotic cell cycle progression in metazoan. However, the sequence and functions of theAnillingene have not been yet characterized in the silkworm,Bombyx mori. In this study, we cloned the full-length cDNA sequence of the silkwormAnillin(BmAnillin) gene. The deduced amino acid sequence for BmAnillin protein comprises an Anillin homology region (AHR) covering an Anillin homology domain and a pleckstrin homology domain. Phylogenetic analysis and multiple alignments of theAnillingenes from silkworm and other organisms indicated evolutionary conservation in the AHR containing conserved phosphorylation sites. Reverse transcription-PCR experiments confirmed that theBmAnillingene was highly expressed during larval development of gonads in which cells undergo mitotic cycles and exhibited an unexpected high expression in silk gland with endocycle during larval molting. RNA interference-mediated knockdown of theBmAnillingene in silkworm BmN4-SID1 cells derived from ovary disrupted chromosome separation and resulted in a loss of the F-actin filament at cleavage furrow during anaphase, suggesting that theBmAnillingene is essential for cytokinesis in silkworm.