LOCATION OF THE RIGHT BOUNDARY OF THE VIRULENCE REGION ON AGROBACTERIUM-TUMEFACIENS PLASMID PTIC58 AND A HOST-SPECIFYING GENE NEXT TO THE BOUNDARY
LOCATION OF THE RIGHT BOUNDARY OF THE VIRULENCE REGION ON AGROBACTERIUM-TUMEFACIENS PLASMID PTIC58 AND A HOST-SPECIFYING GENE NEXT TO THE BOUNDARY
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DOI：
10.1128/jb.168.1.237-243.1986
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发表时间：
1986-10-01
影响因子：
3.2
通讯作者：
KADO, CI
中科院分区：
文献类型：
--
作者：
HIROOKA, T;KADO, CI
通过转座子插入、插入盒定位和缺失突变，确定了根癌农杆菌胭脂碱质粒pTiC58毒力区的右边界。与突变体和野生型等位基因的遗传互补导致在右边界鉴定维雷基因座，其位于从转移到植物基因组中的DNA片段的左边界起约6个酶。维雷长2.0个碱基，编码至少一种69千道尔顿的蛋白质。维雷中的各种突变导致69千道尔顿蛋白质的不同截短长度。随着该蛋白从羧基末端逐渐截短，A.肿瘤形成的频率也随之降低。因此，作为其功能之一，维雷的69kD蛋白可能参与了A.根癌农杆菌的侵染效率。
The right boundary of the virulence (Vir) region of the nopaline plasmid pTiC58 of Agrobacterium tumefaciens was determined by transposon insertion, cartridge emplacement, and deletion mutagenesis. Genetic complementation with mutant and wild-type alleles led to the identification of the virE locus at the right boundary, which was located about 6 kilobases from the left border of the segment of DNA that is transferred into the plant genome. virE is 2.0 kilobases long and encodes at least one protein of 69 kilodaltons. Various mutations in virE resulted in different truncated lengths of the 69-kilodalton protein. As this protein was increasingly truncated from the carboxy terminus, the host range of A. tumefaciens and the frequency of tumor formation diminished concomitantly. Thus, as one of its functions, the 69-kilodalton protein of virE is probably involved in some aspect of the host range specificity of A. tumefaciens and in infection efficiency.