Mutations that affect utilization of a promoter in stationary-phase Bacillus subtilis.
Mutations that affect utilization of a promoter in stationary-phase Bacillus subtilis.
复制标题
影响静止期枯草芽孢杆菌启动子利用的突变。
DOI：
10.1128/jb.163.2.610-614.1985
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发表时间：
1985
影响因子：
3.2
通讯作者：
MoranJr,CP
中科院分区：
文献类型：
--
作者：
Ray,C;Hay,RE;Carter,HL;MoranJr,CP
枯草芽孢杆菌中 ctc 基因的转录仅在指数生长的细胞进入稳定期后才被激活。 ctc 基因的启动子在体外被两种次要形式的 RNA 聚合酶 E sigma 37 和 E sigma 32 利用，但不被最丰富形式的 RNA 聚合酶 E sigma 55 利用。我们使用 ctc 启动子指导质粒 pLC4 上 xylE 基因的转录，并观察到 xylE 仅在稳定期枯草芽孢杆菌中表达。我们还构建了一系列同源质粒，其区别仅在于 ctc 启动子中的特定碱基替换。我们观察到影响 ctc 启动子体内利用（xylE 表达）的碱基取代与我们之前显示的影响 E sigma 37 和 E sigma 32 体外利用启动子的碱基取代相同。我们得出结论，ctc 启动子很可能在体内被 E sigma 37 或 E sigma 32 利用。
Transcription of the ctc gene in Bacillus subtilis is activated only after exponentially growing cells enter stationary phase. The promoter of the ctc gene is utilized in vitro by two minor forms of RNA polymerase, E sigma 37 and E sigma 32, but not by the most abundant form of RNA polymerase, E sigma 55. We have used the ctc promoter to direct transcription of the xylE gene on plasmid pLC4 and observed that xylE was expressed only in stationary-phase B. subtilis. We also have constructed a series of homologous plasmids that differ only by specific base substitutions in the ctc promoter. We observed that the base substitutions that affected utilization of the ctc promoter in vivo (xylE expression) were the same as those that we had previously shown to affect utilization of the promoter in vitro by E sigma 37 and E sigma 32. We conclude that it is likely that the ctc promoter is utilized in vivo by E sigma 37 or E sigma 32.