Photochemical Immobilization of Cells onto a Glass Substrate for in situ DNA Analysis
Photochemical Immobilization of Cells onto a Glass Substrate for in situ DNA Analysis
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DOI：
10.2116/analsci.28.537
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发表时间：
2012-06-01
影响因子：
1.6
通讯作者：
Sato, Kae
中科院分区：
文献类型：
--
作者：
Sasaki, Naoki;Isu, Anri;Sato, Kae
提出了一种将细胞固定到玻璃基板上的简单而可靠的方法。该方法利用二苯甲酮与细胞的光化学反应，二苯甲酮使用标准硅烷偶联剂在基底上进行了修饰。将细胞固定到倒置显微镜下用来自标准光源的紫外光照射的区域。研究了固定对光功率强度和照射时间的依赖性。使用靶标引发的滚环扩增和荧光检测证明了固定化细胞内的原位 DNA 分析。
A simple and robust method to immobilize cells onto a glass substrate is presented. The method employs a photochemical reaction of benzophenone, which is modified on the substrate using a standard silane coupling agent, with cells. Cells were immobilized to an area irradiated with UV light from a standard light source under an inverted microscope. The dependence of immobilization on the light power intensity and irradiation time was investigated. In situ DNA analysis within the immobilized cells was demonstrated using target-primed rolling circle amplification and fluorescent detection.