Recognition of a human sperm surface protein involved in the progesterone-initiated acrosome reaction by antisera against an endomembrane progesterone binding protein from porcine liver.
Recognition of a human sperm surface protein involved in the progesterone-initiated acrosome reaction by antisera against an endomembrane progesterone binding protein from porcine liver.
复制标题
通过抗猪肝内膜黄体酮结合蛋白的抗血清识别参与黄体酮引发的顶体反应的人类精子表面蛋白。
DOI：
10.1016/s0303-7207(99)00173-2
复制
发表时间：
1999
影响因子：
4.1
通讯作者：
Meizel,S
中科院分区：
文献类型：
--
作者：
Buddhikot,M;Falkenstein,E;Wehling,M;Meizel,S
猪肝内膜黄体酮 (P4) 结合蛋白的抗血清可抑制 P4 引发的顶体反应 (AR)，但不能抑制人精子中离子霉素引发的 AR。间接免疫荧光研究检测到精子头部中的抗原，该抗原在获能过程中从后头部区域移动到中头部区域。此外，抗血清在精子洋地黄皂苷提取物的蛋白质印迹中检测到 44.6 kDa 的蛋白质。这些结果表明，与肝内膜 P4 结合蛋白至少部分同源的精子蛋白是精子质膜上参与 P4 启动 AR 的推定 P4 受体。
Antisera against a porcine liver endomembrane progesterone (P4)-binding protein inhibited the P4-initiated acrosome reaction (AR) but not the ionomycin-initiated AR of human sperm. Indirect immunofluorescence studies detected antigen in the sperm head that moved during capacitation from a posterior head region to a midhead region. Moreover, the antisera detected a 44.6 kDa protein in western blots of sperm digitonin extracts. These results suggest that a sperm protein with at least partial homology to the liver endomembrane P4-binding protein, is a putative P4-receptor on the sperm plasma membrane involved in the P4-initiated AR.