Precipitation of DNA with Isopropanol.
Precipitation of DNA with Isopropanol.
复制标题
DOI：
10.1101/pdb.prot093385
复制
发表时间：
2017-08-01
影响因子：
--
通讯作者：
Sambrook, Joseph
中科院分区：
文献类型：
--
作者：
Green, Michael R;Sambrook, Joseph
DNA在含有异丙醇的溶液中比在含有乙醇的溶液中更难溶解。用乙醇沉淀需要2-3体积的酒精，而用异丙醇沉淀需要0.6-0.7体积的酒精。当从大量溶液中沉淀DNA时，异丙醇通常是更好的选择。这里描述的异丙醇沉淀是在室温下进行的，以减少像蔗糖或氯化钠这样的溶质与DNA共沉淀的风险。
DNA is less soluble in solutions containing isopropanol than in solutions containing ethanol. In contrast to precipitation with ethanol, which requires 2-3 volumes of alcohol, precipitation with isopropanol is performed with 0.6-0.7 volume of alcohol. Isopropanol is often the better choice when precipitating DNA from large volumes of solution. Precipitation with isopropanol, described here, is performed at room temperature to lessen the risk that solutes like sucrose or sodium chloride will be coprecipitated with the DNA.