From proteomic inventory to architecture
From proteomic inventory to architecture
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DOI：
10.1016/j.febslet.2004.10.102
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发表时间：
2005-02-07
期刊：
影响因子：
3.5
通讯作者：
Baumeister, W
中科院分区：
文献类型：
--
作者：
Baumeister, W
电子断层扫描可以提供分子分辨率的大型多形性结构的三维重建。虽然电子断层扫描的原理已经知道了几十年，但它的使用只是在最近几年才获得动力。技术进步使其能够应用于冰包生物材料（冷冻断层扫描），从而确保样品的近生命保存。结合先进的计算方法，如基于模式识别的分子鉴定，它是一种很有前途的方法，可以全面绘制细胞器和细胞内的大分子结构，并可视化大分子在其自然环境中的工作。(C)2004年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
Electron tomography can provide three-dimensional reconstructions of large pleomorphic structures at molecular resolution. While the principles of electron tomography have been known for decades, its use has gathered momentum only in recent years. Technological advances have made it possible to apply it to ice-embedded biological material (cryotomography), thereby ensuring a close-to-life preservation of the samples. In combination with advanced computational methods, such as molecular identification based on pattern recognition, it is a promising approach to comprehensively map macromolecular architecture inside organelles and cells and to visualize macromolecules at work in their natural environment. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.