MOLECULAR EVOLUTION OF THE MYOSIN FAMILY - RELATIONSHIPS DERIVED FROM COMPARISONS OF AMINO-ACID-SEQUENCES
MOLECULAR EVOLUTION OF THE MYOSIN FAMILY - RELATIONSHIPS DERIVED FROM COMPARISONS OF AMINO-ACID-SEQUENCES
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DOI：
10.1073/pnas.90.2.659
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发表时间：
1993-01-15
影响因子：
11.1
通讯作者：
SPUDICH, JA
中科院分区：
文献类型：
--
作者：
GOODSON, HV;SPUDICH, JA
为了研究肌球蛋白家族成员之间的进化关系，我们使用了两种不同的系统发育方法，距离矩阵法和最大简约法，分析了所有可用的肌球蛋白头部序列。我们发现至少有三种肌球蛋白有相同的不同分类，这表明目前肌球蛋白的分类只有两类需要重新检验。在肌球蛋白II类中，平滑肌肌球蛋白与非肌肉肌球蛋白的亲缘关系比与横纹肌肌球蛋白的亲缘关系更近，这意味着肌球蛋白和骨骼肌肌球蛋白独立起源于非肌肉肌球蛋白，并提示这些类型的肌肉之间的相似性是趋同进化的结果。通过系统发育分析产生的头部序列的分组与基于酶学和结构定位的分类一致，并且总体上与基于共同的尾部结构元素的分组一致。这一结果表明，在整个进化过程中，特定的头部序列与特定的尾部序列紧密耦合，并挑战了肌球蛋白头部是可自由互换的单位的观点，其独特功能仅由其附着的尾部结构决定。
To examine the evolutionary relationships between members of the myosin family, we have used two different phylogenetic methods, distance matrix and maximum parsimony, to analyze all available myosin head sequences. We find that there are at least three equally divergent classes of myosin, demonstrating that the current classification of myosin into only two classes needs to be reexamined. In the myosin II class, smooth muscle myosin is more closely related to non-muscle myosin than to striated muscle myosin, implying that smooth muscle and skeletal muscle myosins were independently derived from nonmuscle myosin and suggesting that similarities between these types of muscle are the result of convergent evolution. The grouping of head sequences produced by phylogenetic analysis is consistent with classifications based on enzymology and structural localization and is generally consistent with grouping based on common tail structure elements. This result demonstrates that specific head sequences are tightly coupled to specific tail sequences throughout evolution and challenges the idea that myosin heads are freely interchangeable units whose unique function is determined only by the tail structure to which it is attached.