Laccase-mediator system in the decolorization of different types of recalcitrant dyes
Laccase-mediator system in the decolorization of different types of recalcitrant dyes
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DOI：
10.1007/s10295-008-0471-1
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发表时间：
2009-01-01
影响因子：
3.4
通讯作者：
Qian, Shijun
中科院分区：
文献类型：
--
作者：
Hu, Mei Rong;Chao, Ya Peng;Qian, Shijun
间苯三酚，百里酚，和紫尿酸（VIO）被选为漆酶介质后，筛选14个不同的化合物与靛蓝胭脂红（靛蓝染料）作为底物。在这三种介质的存在下，在1小时内几乎完全脱色（90-100%）。因此，这三种化合物被用作其他四种染料的脱色介质。结果表明，VIO对蒽醌类染料雷马唑艳蓝R、三苯甲烷类染料考马斯亮蓝G-250和重氮染料酸性红均有较好的脱色效果。在VIO存在下，上述四种染料达到70%脱色。百里酚能够介导RBBR和天青A（杂环染料）的脱色。间苯三酚对所分析的四种染料的脱色没有介导作用。介质浓度、pH值和铜离子对RBBR的脱色有影响。漆酶/介体体系的脱色能力与介体的种类、染料的结构和脱色条件有关。
Phloroglucinol, thymol, and violuric acid (VIO) were selected as laccase mediators after screening 14 different compounds with indigo carmine (indigoid dye) as a substrate. With the presence of these three mediators, a nearly complete decolorization (90-100%) was attained in 1 h. Thus, these three compounds were used as mediators for the decolorization of other four dyes. The results indicated that VIO was effective mediator in decolorization of Remazol brilliant blue R (RBBR, anthraquinoid dye) and Coomassie brilliant blue G-250 (CBB, triphenylmethane dyes), and Acid red (diazo dye). In presence of VIO, the four dyes described above attained 70% decolorization. Thymol was able to mediate decolorization of RBBR and Azure A (heterocyclic dye). Phloroglucinol has no mediating capability in decolorization of the four dyes analyzed. Mediator concentration, pH, and copper ion have an effect on the decolorization of the RBBR. Our data suggested that the decolorization capabilities of laccase/mediator system were related to the types of mediator, the dye structure and decolorization condition.